Risk assessment of the newly emerged H7N9 avian influenza viruses.
Risk assessment of the newly emerged H7N9 avian influenza viruses.
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新出现的H7N9禽流感病毒的风险评估。
DOI：
10.1080/22221751.2023.2172965
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发表时间：
2023-12
影响因子：
13.2
通讯作者：
中科院分区：
文献类型：
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作者：
自2013年首例人感染以来，H7N9禽流感病毒(AIV)已导致1500多人感染，死亡率约为40%。尽管中国各地实施了大规模家禽疫苗接种制度，但H7N9禽流感病毒仍在家禽中持续存在并迅速演变。最近，已分离出几株H7N9禽流感病毒，并显示出逃避疫苗诱导免疫的能力。为了评估最近分离的H7N9禽流感病毒的人畜共患病风险，我们从这些H7N9禽流感病毒和PR8病毒的6个内部片段中回收了血凝素(HA)和神经氨酸酶(NA)的病毒，并对它们的受体结合、融合pH、热稳定性、空斑形态和卵内病毒复制进行了研究。我们还评估了病毒与人抗H7N9 HA的单抗和抗H7N9 AIV候选疫苗的雪貂抗血清的交叉反应性。早期流行波的H7N9禽流感病毒具有双重唾液酸受体结合特征，而最近的H7N9禽流感病毒完全失去或保留了微弱的人唾液酸受体结合。与第一波流行的H7N9禽流感病毒相比，2020/21病毒在Madin-Darby犬肾(MDCK)细胞上形成了更大的斑块，并在卵子中复制到更高的滴度，表现出更高的酸稳定性，但热稳定性降低。进一步分析表明，这些最近的H7N9禽流感病毒与人mAbs和雪貂抗血清的交叉反应性较差，突显了更新候选疫苗的必要性。总之，新出现的H7N9禽流感病毒具有典型禽流感病毒的特征，人畜共患病风险降低，但对家禽的威胁增加。
Since the first human case in 2013, H7N9 avian influenza viruses (AIVs) have caused more than 1500 human infections with a mortality rate of approximately 40%. Despite large-scale poultry vaccination regimes across China, the H7N9 AIVs continue to persist and evolve rapidly in poultry. Recently, several strains of H7N9 AIVs have been isolated and shown the ability to escape vaccine-induced immunity. To assess the zoonotic risk of the recent H7N9 AIV isolates, we rescued viruses with hemagglutinin (HA) and neuraminidase (NA) from these H7N9 AIVs and six internal segments from PR8 virus and characterized their receptor binding, pH of fusion, thermal stability, plaque morphology and in ovo virus replication. We also assessed the cross-reactivity of the viruses with human monoclonal antibodies (mAbs) against H7N9 HA and ferret antisera against H7N9 AIV candidate vaccines. The H7N9 AIVs from the early epidemic waves had dual sialic acid receptor binding characteristics, whereas the more recent H7N9 AIVs completely lost or retained only weak human sialic acid receptor binding. Compared with the H7N9 AIVs from the first epidemic wave, the 2020/21 viruses formed larger plaques in Madin-Darby canine kidney (MDCK) cells and replicated to higher titres in ovo, demonstrating increased acid stability but reduced thermal stability. Further analysis showed that these recent H7N9 AIVs had poor cross-reactivity with the human mAbs and ferret antisera, highlighting the need to update the vaccine candidates. To conclude, the newly emerged H7N9 AIVs showed characteristics of typical AIVs, posing reduced zoonotic risk but a heightened threat for poultry.
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影响因子：
5.4
作者：
通讯作者：
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影响因子：
5.4
作者：
Bhat S;James J;Sadeyen JR;Mahmood S;Everest HJ;Chang P;Walsh SK;Byrne AMP;Mollett B;Lean F;Sealy JE;Shelton H;Slomka MJ;Brookes SM;Iqbal M
通讯作者：
Iqbal M
DOI：
10.3390/v10020081
发表时间：
2018-02-13
期刊：
Viruses
影响因子：
--
作者：
Chang P;Yao Y;Tang N;Sadeyen JR;Sealy J;Clements A;Bhat S;Munir M;Bryant JE;Iqbal M
通讯作者：
Iqbal M
影响因子：
28.3
作者：
Huang, Kuan-Ying A.;Rijal, Pramila;Townsend, Alain R.
通讯作者：
Townsend, Alain R.
影响因子：
5.4
作者：
Reed, Mark L.;Bridges, Olga A.;Russell, Charles J.
通讯作者：
Russell, Charles J.