Value of ethyl glucuronide in plasma as a biomarker for recent alcohol consumption in the emergency room
Value of ethyl glucuronide in plasma as a biomarker for recent alcohol consumption in the emergency room
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DOI：
10.1093/alcalc/agn035
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发表时间：
2008-07-01
影响因子：
2.8
通讯作者：
Spies, Claudia
中科院分区：
文献类型：
--
作者：
Neumann, Tim;Helander, Anders;Spies, Claudia
目的：本急诊科（ED）研究比较了血浆乙基葡萄糖醛酸苷（EtG）检测与标准酒精生物标志物-谷氨酰转移酶（GGT）和碳水化合物缺乏转移（CDT）以及审计问卷获得的酒精消耗信息的价值。方法：采用计算机化审计问卷和纸笔评估（包括酒精摄入的类型、数量和时间），对入住急诊科的轻度损伤和临床未醉酒的男性患者（n = 81）进行酒精摄入筛查。采集血样，测定血浆中乙醇、EtG （LC-MS）、GGT和血清中CDT （Axis-Shield %CDT免疫分析法）的含量。结果：81例患者中，23例（28%）的审计问卷呈阳性（>= 8分）。只有3个（4%）检测到乙醇浓度（0.01 ~ 0.07 g/L）， 31个（38%）检测到EtG （0.16 ~ 39.5 mg/L）。在4例患者中，在估计完全消除乙醇后48小时血浆中可检测到EtG。EtG与长期生物标志物%CDT或GGT或审计结果无关，但与估计完成乙醇消除的时间有关。结论：血液中EtG测试在ED中被发现是一种有用的方法，可以检测最近饮酒，即使在酒精测试阴性的情况下，也可以确认戒酒。这种敏感而特异的短期生物标志物提供了关于个人饮酒习惯的有价值的额外信息，也可能有助于识别酒精宿醉。
Aim: This emergency department (ED) study compared the value of plasma ethyl glucuronide (EtG) testing with the information about alcohol consumption obtained using the standard alcohol biomarkers gamma-glutamyltransferase (GGT) and carbohydrate-deficient transferring (CDT) and the AUDIT questionnaire. Methods: Minimally injured and clinically non-intoxicated male patients (n = 81) admitted to an ED were screened regarding their alcohol consumption, using the computerized AUDIT questionnaire and a paper-and-pencil assessment including the type, amount and time of alcohol intake. Blood samples were collected for determination of ethanol, EtG (LC-MS) and GGT in plasma and %CDT in serum (Axis-Shield %CDT immunoassay). Results: Out of the 81 patients, 23 (28%) were positive (>= 8 points) on the AUDIT questionnaire. Only 3 (4%) showed a detectable ethanol concentration (range 0.01-0.07 g/L) but 31 (38%) showed a detectable EtG (0.16-39.5 mg/L). In four patients, EtG was detectable in plasma for > 48 h after estimated completed elimination of ethanol. EtG was not correlated with the long-term biomarkers %CDT or GGT, or the AUDIT results, but with the time since estimated completed ethanol elimination. Conclusion: EtG testing in blood was found useful in the ED as a way to detect recent drinking, even in cases of a negative ethanol test, and to confirm abstinence from alcohol. This sensitive and specific short-term biomarker provides valuable additional information about individual drinking habits and might also be helpful to identify an alcohol hangover.