Expressed sequence tags from cynomolgus monkey (Macaca fascicularis) liver:: A systematic identification of drug-metabolizing enzymes
Expressed sequence tags from cynomolgus monkey (Macaca fascicularis) liver:: A systematic identification of drug-metabolizing enzymes
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DOI：
10.1016/j.febslet.2007.12.031
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发表时间：
2008-01-01
期刊：
影响因子：
3.5
通讯作者：
Inoue, Ituro
中科院分区：
文献类型：
--
作者：
Uno, Yasuhiro;Suzuki, Yutaka;Inoue, Ituro
肝脏是药物代谢的主要器官，猴子和人类的肝脏在生理上是相似的。然而，鉴定基因的缺乏阻碍了对猴子药物代谢的深入了解。为了提供这样的遗传资源，从食蟹猴肝脏全长富集cDNA文库中获得28 655个表达序列标签（est），其中包含23个与人类药物代谢酶同源的独特est。我们的比较基因组学方法确定了9种谱系特异性候选ESTs，包括3种药物代谢酶，这对于理解猴子和人类之间的生理差异可能很重要。(C) 2007年欧洲生化学会联合会。Elsevier B.V.版权所有。
The liver, a major organ for drug metabolism, is physiologically similar between monkeys and humans. However, the paucity of identified genes has hampered a deep understanding of drug metabolism in monkeys. To provide such a genetic resource, 28 655 expressed sequence tags (ESTs) were generated from a cynomolgus monkey liver full-length enriched cDNA library, which contained 23 unique ESTs homologous to human drug-metabolizing enzymes. Our comparative genomics approach identified nine lineage-specific candidate ESTs, including three drug-metabolizing enzymes, which could be important for understanding the physiological differences between monkeys and humans. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.