Decreasing pH of rat embryos and fluids estimated by transplacental distribution of DMO.
Decreasing pH of rat embryos and fluids estimated by transplacental distribution of DMO.
复制标题
通过 DMO 经胎盘分布估计大鼠胚胎和体液 pH 值降低。
DOI：
10.1152/ajpregu.1989.257.3.r542
复制
发表时间：
1989
期刊：
影响因子：
--
通讯作者：
ScottJr,WJ
中科院分区：
文献类型：
--
作者：
Collins,MD;Duggan,CA;Schreiner,CM;ScottJr,WJ
利用弱酸5，5-二甲基恶唑烷-2，4-二酮(DMO)经胎盘的分布，我们测量了妊娠11.5-14天大鼠胚胎体内细胞的pH值。在这个物种中，这是一个快速器官发生的时期，许多器官系统的细胞开始从增殖模式转变为分化状态。结果发现，胎龄为11.5d的大鼠胚胎细胞内pH为7.47+/-0.03，14d时细胞内pH值为7.11+/-0.03。因为在发育过程中伴随着增殖率的下降，我们认为这种相关性是细胞增殖和细胞内部碱化之间联系的额外证据。包括胚胎血浆、羊水、体外液和卵黄囊在内的其他几个隔室的DMO浓度随着发育的进展而下降，表明pH值稳步下降。这些变化可能会对发育和药代动力学产生影响。
Utilizing the transplacental distribution of a weak acid, 5,5-dimethyloxazolidine-2,4-dione (DMO), we have measured the pH of cells within the rat embryo in vivo on days 11.5-14 of gestation. This is a period of rapid organogenesis in this species when the cells of many organ systems begin to change from a proliferative mode into a differentiated state. We found that intracellular pH of the day 11.5 rat embryo is 7.47 +/- 0.03 and decreases steadily to day 14 at which time it reaches 7.11 +/- 0.03. Because there is a concomitant fall in proliferative rate over this span of development, we suggest this correlation to be additional evidence of an association between proliferation and alkalinization of the cell interior. A number of other compartments including embryo plasma, amniotic fluid, exocoelomic fluid, and yolk sac have a decreasing concentration of DMO as development advances, indicative of a steadily declining pH. These changes could have developmental and pharmacokinetic implications.