Histone H4 acetylation of euchromatin and heterochromatin is cell cycle dependent and correlated with replication rather than with transcription
Histone H4 acetylation of euchromatin and heterochromatin is cell cycle dependent and correlated with replication rather than with transcription
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DOI：
10.1105/tpc.12.11.2087
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发表时间：
2000-11-01
期刊：
影响因子：
11.6
通讯作者：
Schubert, I
中科院分区：
文献类型：
--
作者：
Jasencakova, Z;Meister, A;Schubert, I
核小体组蛋白H3和H4的可逆乙酰化通常被认为与真核染色质结构域的潜在转录活性相关。在这里，我们报告的H4乙酰化的程度内常染色质和异染色质结构域与DNA复制，而不是与转录活性，而H3乙酰化在整个细胞周期中保持相当恒定。与常染色质相比，植物核仁组织者在有丝分裂期间在H4处更强烈地乙酰化，但在S期期间乙酰化较少，此时核仁似乎（至少暂时）没有核小体。H4的赖氨酸5和12的沉积相关乙酰化似乎在动物和植物中是保守的，并且在植物中延伸到K16。在4 '，6-二脒基-2-苯基吲哚（DAPI）染色的异染色质组分中，H3的赖氨酸9/18和14处出现了可能的物种特异性高于平均水平的乙酰化。这些结果是通过结合免疫检测H3和H4的所有乙酰化亚型有丝分裂染色体和细胞核在G1，早期S，中期S，晚期S，和G2期的领域豆与识别特定的染色质结构域的荧光原位杂交或DAPI染色。此外，不同结构域的组蛋白乙酰化模式与它们的复制和转录模式进行了比较。
Reversible acetylation of nucleosomal histones H3 and H4 generally is believed to be correlated with potential transcriptional activity of eukaryotic chromatin domains. Here, we report that the extent of H4 acetylation within euchromatin and heterochromatic domains is linked with DNA replication rather than with transcriptional activity, whereas H3 acetylation remains fairly constant throughout the cell cycle. Compared with euchromatin, plant nucleolus organizers were more strongly acetylated at H4 during mitosis but less acetylated during S phase, when the nucleolus appeared to be (at least transiently) devoid of nucleosomes. Deposition-related acetylation of lysines 5 and 12 of H4 seems to be conserved in animals and plants and extended to K16 in plants. A possibly species-specific above-average acetylation at lysines 9/18 and 14 of H3 appeared in 4',6-diamidino-2-phenylindole (DAPI)-stained heterochromatin fractions. These results were obtained by combining immunodetection of all acetylatable isoforms of H3 and H4 on mitotic chromosomes and nuclei in G1, early S, mid-S, late S, and G2 phases of the field bean with identification of specific chromatin domains by fluorescence in situ hybridization or DAPI staining. In addition, the histone acetylation patterns of distinct domains were compared with their replication and transcription patterns.