Ethylene mediates dichromate-induced oxidative stress and regulation of the enzymatic antioxidant system-related transcriptome in Arabidopsis thaliana
Ethylene mediates dichromate-induced oxidative stress and regulation of the enzymatic antioxidant system-related transcriptome in Arabidopsis thaliana
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DOI：
10.1016/j.envexpbot.2018.09.004
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发表时间：
2019-05
影响因子：
5.7
通讯作者：
A. Wakeel;I. Ali;Minjie Wu;Ali Raza Kkan;Mehmood Jan;Abid Ali;Yihua Liu;Song Ge;Junyu Wu;Bohan Liu;Yinbo Gan
中科院分区：
文献类型：
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作者：
A. Wakeel;I. Ali;Minjie Wu;Ali Raza Kkan;Mehmood Jan;Abid Ali;Yihua Liu;Song Ge;Junyu Wu;Bohan Liu;Yinbo Gan
重铬酸盐 [Cr(VI)] 通过直接干扰植物的生理、生化和/或分子过程或通过刺激活性氧积累 (ROS) 间接诱导植物毒性。 Cr(VI) 诱导的 ROS 介导的植物毒性的潜在机制尚不清楚。在当前的研究中，我们研究了 Cr(VI) 诱导的总生物量产生、细胞/组织活力和细胞周期相关转录组的改变是由高 ROS 水平的积累介导的可能性，并且乙烯参与了这一过程。我们发现，高 Cr(VI) 水平会降低总生物量、叶绿素含量、细胞/组织活力以及细胞周期相关基因的表达。一致地，暴露于较高 Cr(VI) 浓度的幼苗的氧化损伤和 ROS 积累显着增加。此外，本研究还报道了 Cr(VI) 诱导的 EBS::GUS 活性以及乙烯信号和生物合成相关基因的表达水平。此外，通过下调酶抗氧化系统相关基因，Cr(VI)诱导的ROS积累因外源乙烯前体(ACC)的应用而略有增加，而乙烯感知和生物合成拮抗剂银(Ag)和钴(Co)分别显着减少。综上所述，我们的结果表明，乙烯通过抑制酶抗氧化酶相关基因的表达水平来介导重铬酸盐诱导的ROS积累。
Dichromate [Cr(VI)] induces phytotoxicity either directly by interfering in a plant’s physiological, biochemical, and/or molecular process or indirectly by stimulating reactive oxygen species accumulation (ROS). The underlying mechanism in Cr(VI)-induced ROS mediated phytotoxicity is elusive. In the current study, we investigate the possibility that Cr(VI)-induced alteration in the total biomass production, cell/tissue viability, and cell cycle-related transcriptome is mediated by the accumulation of high ROS level and that ethylene participates in this process. We showed that high Cr(VI) level reduces total biomass, chlorophyll contents, cell/tissue viability, and expression of cell cycle-related genes. Consistently, the oxidative damage and ROS accumulations were significantly increased in the seedlings exposed to higher Cr(VI) concentrations. Furthermore, Cr(VI)-induced EBS::GUS activity and expression level of ethylene signaling and biosynthesis related genes were also reported in the current study. Moreover, Cr(VI)-induced ROS accumulation by down-regulating enzymatic antioxidant system-related genes was slightly increased by the application of exogenous ethylene precursor (ACC), while significantly reduced by ethylene perception and biosynthesis antagonists silver (Ag) and cobalt (Co) respectively. Taken together, our results showed that ethylene mediates dichromate induced ROS accumulation by suppressing the expression level of enzymatic antioxidant enzymes-related genes.