Antibiotic-mediated antagonism leads to a bacterial game of rock-paper-scissors in vivo
Antibiotic-mediated antagonism leads to a bacterial game of rock-paper-scissors in vivo
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DOI：
10.1038/nature02429
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发表时间：
2004-03-25
期刊：
影响因子：
64.8
通讯作者：
Riley, MA
中科院分区：
文献类型：
--
作者：
Kirkup, BC;Riley, MA
Colicins是由大肠杆菌及其近亲生产并对其具有活性的窄谱抗生素。产生结肠素的菌株不能与敏感或耐药菌株在良好的混合培养中共存，但所有这三种表型都可以在自然种群中恢复(1)。最近的体外结果表明，在空间结构的、非传递性的相互作用中，粘菌素的产生可以促进菌株的多样性(2)，就像经典的非传递性模型岩石-布-剪刀(RPS)一样。在粘菌素版本的RPS模型中，产生粘菌素(C)的菌株杀死敏感(S)菌株，后者比耐药(R)菌株竞争，后者比C菌株竞争。这三个菌株之间的体外竞争按可预测的顺序解决(C击败S，S击败R，R击败C)，但三个菌株的完整系统提供了动态平衡的机会(2)。在这里，我们提供了粘菌素在体内发挥拮抗作用的确凿证据，并表明粘菌素(以及潜在的其他细菌素)可能促进而不是消除环境中的微生物多样性。
Colicins are narrow-spectrum antibiotics produced by and active against Escherichia coli and its close relatives. Colicin-producing strains cannot coexist with sensitive or resistant strains in a well-mixed culture, yet all three phenotypes are recovered in natural populations(1). Recent in vitro results conclude that strain diversity can be promoted by colicin production in a spatially structured, non-transitive interaction(2), as in the classic nontransitive model rock - paper - scissors (RPS). In the colicin version of the RPS model, strains that produce colicins (C) kill sensitive (S) strains, which outcompete resistant (R) strains, which outcompete C strains. Pairwise in vitro competitions between these three strains are resolved in a predictable order ( C beats S, S beats R, and R beats C), but the complete system of three strains presents the opportunity for dynamic equilibrium(2). Here we provide conclusive evidence of an in vivo antagonistic role for colicins and show that colicins ( and potentially other bacteriocins) may promote, rather than eliminate, microbial diversity in the environment.