INTERACTION OF TRICHOHYALIN WITH INTERMEDIATE FILAMENTS - 3 IMMUNOLOGICALLY DEFINED STAGES OF TRICHOHYALIN MATURATION
INTERACTION OF TRICHOHYALIN WITH INTERMEDIATE FILAMENTS - 3 IMMUNOLOGICALLY DEFINED STAGES OF TRICHOHYALIN MATURATION
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DOI：
10.1111/1523-1747.ep12494172
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发表时间：
1992-01-01
影响因子：
6.5
通讯作者：
MANABE, M
中科院分区：
文献类型：
--
作者：
OGUIN, WM;SUN, TT;MANABE, M
Trichohyalin是一种220 kD的蛋白质，存在于人毛囊的髓质和内根鞘细胞中，作为主要分化产物存在于Trichohyalin颗粒中。目前还不清楚该蛋白是作为内根鞘的中间丝前体，还是作为中间丝相关的(基质)蛋白。我们已经制备了一组针对该蛋白的单抗(AE15-17)，并用它们来追踪它在内根鞘分化过程中的命运。这些研究使我们能够确定这种鞭毛虫蛋白的三种不同的免疫学形式。它们是：1)AE15阳性形式，存在于所有的毛状菌素颗粒中；2)AE16阳性形式，以离散的点状分布在毛状菌素颗粒的表面；以及3)AE17阳性的中间纤维结合型，它与内根鞘纤维相联系，具有规则的400 nm周期。根据这些结果，我们认为220-kD的Trichohyalin蛋白是一种中间丝结合蛋白，可能在内根鞘纤维束的横向聚集、精确排列和稳定中发挥作用。
"Trichohyalin" is a 220-kD protein found in trichohyalin granules that are present as major differentiation products in the medulla and inner root sheath cells of human hair follicles. It was unclear whether this protein served as an intermediate filament precursor in the inner root sheath or as an intermediate-filament-associated (matrix) protein. We have produced a panel of monoclonal antibodies (AE15-17) to this protein and used them to trace its fate during inner root sheath differentiation. These studies have allowed us to define three immunologically distinct forms of this trichohyalin protein. They are 1) the AE15-positive form, which is found throughout all trichohyalin granules; 2) the AE16-positive form, which is localized as discrete punctae on the surface of trichohyalin granules; and 3) the AE17-positive, intermediate-filament-bound form, which associates with the inner root sheath filaments with a regular, 400-nm periodicity. From these results we suggest that the 220-kD trichohyalin protein is an intermediate-filament-asociated protein that may play a role in the lateral aggregation, precise alignment, and stabilization of inner root sheath filament bundles.