Analysis of Immunohistochemical Expression of K19 in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma Using Color Deconvolution-Image Analysis Method
Analysis of Immunohistochemical Expression of K19 in Oral Epithelial Dysplasia and Oral Squamous Cell Carcinoma Using Color Deconvolution-Image Analysis Method
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DOI：
10.1007/s12105-010-0210-6
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发表时间：
2010-12-01
影响因子：
2.1
通讯作者：
Hamasha, Abed Al-Hadi
中科院分区：
文献类型：
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作者：
Safadi, Rima A.;Musleh, Atika S.;Hamasha, Abed Al-Hadi
K19是一种在口腔鳞状细胞癌（OSCC）中研究的中间丝蛋白，但与分化良好的OSCC等级中产生的角蛋白量无关。本研究的目的是客观分析K19在口腔鳞状细胞癌中的表达，并验证K19在口腔上皮异型增生（OED）分级中的鉴别作用。福尔马林固定的组织包括36例原发性OSCC（22例良好，10例中度，4例低分化），43例OED（23例轻度，8例中度，12例重度）和11例正常口腔上皮（NOE）。采用HRP-DAB法对K19进行免疫组化染色。使用ImageJ（R）图像分析系统（公共领域软件，美国马里兰州贝塞斯达的国立卫生研究院（National Institutes of Health，Bethesda，MD，USA））中的彩色去卷积程序发现K19阳性面积的百分比，并使用独立样本t检验和ANOVA检验进行分析。K19评分在NOE、轻度、中度和重度OED中分别为1.8、3.4、21和50.3%，与分级显著相关（t检验P < 0.05）。高分化鳞癌伴角蛋白珠30%（分别为28.6%和1.2%，P < 0.05）。K19评分在中、低分化口腔鳞癌中分别为60.8%和61.3%。K19评分在分化良好的OSCC分级中显著区分两个亚组的肿瘤，并反映组织学分化以及可能预测临床结果。将K19免疫染色与常规H&E染色相结合，有助于更准确地判定OED的分级。
K19 is an intermediate filament protein that has been investigated in oral squamous cell carcinoma (OSCC), but that has not been correlated with the amount of keratin produced within well-differentiated OSCC grade. The aim of the present study was to objectively analyze K19 immunoexpression in OSCC and to validate the utility of K19 in differentiation among grades of oral epithelial dysplasia (OED). Formalin-fixed tissues of 36 primary OSCC (22 well, 10 moderately, 4 poorly differentiated), 43 OED (23 mild, 8 moderate, 12 severe), and 11 normal oral epithelium (NOE) were included. K19 was immunostained using HRP-DAB method. The percentage of K19-positive areawas found using color deconvolution program in ImageJ (R) image analysis system(public domain software, National Institutes of Health, Bethesda, MD, USA) and analyzed using independent samples t tests and ANOVA test. K19 scores in NOE, mild, moderate and severe OED were: 1.8, 3.4, 21, and 50.3%, respectively, with significant association with the grade (t test P < 0.05). Well-differentiated OSCC with 30% keratin pearls (28.6 and 1.2%, respectively, P < 0.05). K19 scores in moderately and poorly differentiated OSCC were 60.8 and 61.3%, respectively. K19 scores significantly differentiated between two subgroups of tumors within well-differentiated OSCC grade and reflected histologic differentiation aswell as probably predicting the clinical outcome. Combining K19 immunostain with the regular H&E stain may be helpful to facilitate and assure assigning amore accurate grade forOED.