LOSS OF IMMUNOREACTIVITY IN LONG-TERM BONE-MARROW CULTURE
LOSS OF IMMUNOREACTIVITY IN LONG-TERM BONE-MARROW CULTURE
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DOI：
10.1038/275135a0
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发表时间：
1978-01-01
期刊：
影响因子：
64.8
通讯作者：
SPOONCER, E
中科院分区：
文献类型：
--
作者：
DEXTER, TM;SPOONCER, E
我们已经开发了一种长期骨髓培养系统，其中表面上功能正常的造血干细胞（CFU-S）的增殖和分化可以在体外维持数月1 -8。我们现在发现，遗传抗性9，10或宿主抗移植物（H-v-G）反应，（可能）相关的自然杀伤（NK）细胞活性11和培养细胞重建辐射同种异体（不相容）受体（随后发生G-v-H疾病12）的能力都不存在于这些培养细胞中。
WE have developed a long-term bone marrow culture system in which proliferation and differentiation of apparently functionally normal haemopoietic stem cells (CFU-S) can be sustainedin vitrofor several months1–8. We now find that genetic resistance9,10or host-versus-graft (H-v-G) reaction, the (possibly) related natural killer (NK) cell activity11and the ability of cultured cells to reconstitute irradiated allogeneic (non-compatible) recipients (with the subsequent development of G-v-H disease12) are all absent from such cultured cells.