RELAXATION OF RECOGNITION SEQUENCE OF SPECIFIC ENDONUCLEASE HINDIII
RELAXATION OF RECOGNITION SEQUENCE OF SPECIFIC ENDONUCLEASE HINDIII
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DOI：
10.1093/nar/14.2.811
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发表时间：
1986-01-24
影响因子：
14.9
通讯作者：
THOMAS, D
中科院分区：
文献类型：
--
作者：
NASRI, M;THOMAS, D
在标准反应条件下，限制性内切酶HindIII在箭头所示位置切割识别序列AAGCTT-内的双链DNA。在二甲基亚砜的存在下，该酶的底物特异性降低，并且在其他位点发生裂解。我们确定了pBR322 DNA在放松条件下被HindIII内切酶识别的二级位点，发现它在箭头所示的位置切割六核苷酸，产生具有内聚末端的片段。
Under the standard, reaction conditions, the restriction endonuclease HindIII cleaves double-stranded DNA, within the recognition sequence AAGCTT- at the position indicated by the arrow. In the presence of Dimethyl sulfoxide the substrate specificity of this enzyme is reduced and cleavages occur at additional sites.We have determined the secondary sites in pBR322 DNA recognized by HindIII endonuclease under relaxed conditions and found that it cleaves the hexanucleotides:at the positions Indicated by the arrows, producing fragments with cohesive ends.