Improvement of postharvest quality, regulation of antioxidants capacity and softening enzymes activity of cold-stored carambola in response to polyamines application
Improvement of postharvest quality, regulation of antioxidants capacity and softening enzymes activity of cold-stored carambola in response to polyamines application
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DOI：
10.1016/j.postharvbio.2018.10.017
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发表时间：
2019-02-01
影响因子：
7
通讯作者：
Ali, Asgar
中科院分区：
文献类型：
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作者：
Ahmad, Azhane;Ali, Asgar
在杨桃果实冷藏过程中，研究了腐胺（PUT）和亚精胺（SPD）对杨桃果实采后品质保鲜、生物活性物质和细胞壁软化酶的调节作用。B10)。将三种不同浓度（0.0、0.1、1.0和2.0 mM）的PUT和SPD施于果实5 min后，在3℃、85- 90% RH条件下贮存28 d。最高浓度（2.0 mM）的PUT有效地减少了果实失重，保持果皮颜色（L*、C*、h度），保持了较高的硬度，降低了呼吸速率和乙烯释放量，并抑制了整个贮存过程中可溶性固溶体含量（SSC）和可滴定酸度（TA）的降解。此外，2.0 mM PUT处理延缓了β -半乳糖苷酶（β -gal）、聚半乳糖醛酸酶（PG）和果胶甲基酯酶（PME）酶活性的进展，同时果实硬度的变化不太明显。抗坏血酸（AA）、总抗氧化剂含量（TAC）和酚类物质含量（TPC）在处理后均显著降低，而对照果实则显著降低。最高浓度（2.0 mM）的PUT和SPD通过增加TPC来提高TAC活性的效果最好。较低浓度SPD （1.0 mM和0.1 mM）刺激AA含量升高。这一发现揭示了多胺（PAs）作为维持B10杨桃采后品质、增强抗氧化能力和降低软化酶活性的有效手段，具有延长杨桃货架期的潜力。
The efficacy of putrescine (PUT) and spermidine (SPD) application in preserving postharvest quality, regulating bioactive compounds and cell wall softening enzymes was examined during cold storage of carambola fruit (Averrhoa carambola cv. B10). Three different concentrations (0.0, 0.1, 1.0 and 2.0 mM) of PUT and SPD were applied to fruit by immersion for 5 min and further stored at 3 degrees C, 85-90 % RH for 28 d. The highest concentration (2.0 mM) of PUT effectively reduced weight loss, maintained peel colour (L*, C*, h degrees), retained higher firmness, lowered respiration rate and ethylene emission, and suppressed the degradation of soluble solids content (SSC) and titrable acidity (TA) throughout storage. Moreover, 2.0 mM PUT treatment delayed the advancement of beta-galactosidase (beta-gal), polygalacturonase (PG) and pectin methylesterase (PME) enzymes activity concomitant with less apparent changes in fruit firmness. Ascorbic acid (AA), total antioxidant content (TAC) and phenolic content (TPC) were enhanced to higher levels in treated fruit, while control fruit experienced significant loss of these bioactive compounds. The highest concentration (2.0 mM) of PUT and SPD exhibited the best result of increasing TAC activity through an increment of TPC. In contrast, higher AA content was stimulated by lower concentration of SPD (1.0 and 0.1 mM). This present finding throws light on the potential of polyamines (PAs) as an effective mean for maintaining postharvest quality, enhancing antioxidants capacity and provokes reduction on softening enzymes activity, with the prolong shelf-life of B10 carambola.