Isolation of infectious salmon anemia virus (ISAV) from Atlantic salmon in New Brunswick, Canada
Isolation of infectious salmon anemia virus (ISAV) from Atlantic salmon in New Brunswick, Canada
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DOI：
10.3354/dao035131
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发表时间：
1999-01-29
影响因子：
1.4
通讯作者：
Nicholson, BL
中科院分区：
文献类型：
--
作者：
Bouchard, D;Keleher, W;Nicholson, BL
传染性鲑鱼贫血病毒（ISAV）是在加拿大新玩法的一个海洋生长点从大西洋鲑鱼萨尔莫中分离的，该鲑鱼由于出血性肾综合征（HKS）而死亡。在本研究中取样的20条鱼中，14条在组织学上表现出不同程度的间质增生、肾小管上皮变性和坏死以及后肾中的肾小管管型，这是典型的HKS。接种后10 ~ 14 d，后肾和脾匀浆对大鳞鲑胚胎（CHSE-214）细胞产生致细胞病变作用。通过电子显微镜观察到大小范围为80至120 nm的多形病毒颗粒。用逆转录-聚合酶链反应（RT-PCR）证实该病毒为ISAV。这是一个系统的诊断研究分离ISAV在北美大陆和生长的ISAV的CHSE-214细胞系的第一个描述。
Infectious salmon anemia virus (ISAV) was isolated at a marine grow-out site in New Brunswick, Canada, from Atlantic salmon Salmo salar which experienced mortalities due to hemorrhagic kidney syndrome (HKS). Of 20 fish sampled in this study, 14 showed histologically various degrees of interstitial hemorrhaging, tubular epithelial degeneration and necrosis, and tubular casts in the posterior kidney, typical of HKS. Posterior kidney and spleen homogenates produced a cytopathic effect on chinook salmon embryo (CHSE-214) cells 10 to 14 d after inoculation. Pleomorphic virus particles in the size range 80 to 120 nm were seen by electron microscopy. The virus was confirmed as ISAV using reverse transcriptase-polymerase chain reaction (RT-PCR). This is a systematic diagnostic study of the isolation of ISAV on the North American continent and the first description of the growth of ISAV on the CHSE-214 cell line.