Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach.
Optimization of Differentiation of Nonhuman Primate Pluripotent Cells Using a Combinatorial Approach.
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DOI：
10.1007/978-1-4939-9007-8_14
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发表时间：
2019
影响因子：
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通讯作者：
Steven L. Farnsworth;Zhifang Qiu;A. Mishra;P. Hornsby
中科院分区：
文献类型：
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作者：
Steven L. Farnsworth;Zhifang Qiu;A. Mishra;P. Hornsby
将多能干细胞定向分化为所需的谱系通常涉及复杂和冗长的方案。为了系统地研究分化的要求，我们提出了一种使用小分子和生物因素组合的迭代方法。这些因子在一个循环过程中使用，在该过程中，在一轮测试中选择最佳的因子和浓度组合，然后对组合和后续几轮进行修改。虽然这可能会在研究中的细胞群体中产生所需的分化，但也可能需要其他策略来优化分化过程。这些策略将在本章中介绍。
The directed differentiation of pluripotent stem cells to a desired lineage often involves complex and lengthy protocols. In order to study the requirements for differentiation in a systematic way, we present here methodology for an iterative approach using combinations of small molecules and biological factors. The factors are used in a cyclical process in which the best combination of factors and concentrations is selected in one round of testing, followed by a modification of the combination and subsequent rounds. While this may produce the desired differentiation in the cell population under study, it is also possible that other strategies may be needed to optimize the differentiation process. These strategies are described in this chapter.