DUAL REGULATION OF 21-HYDROXYLASE ACTIVITY BY SEX STEROID-HORMONES IN RAT HEPATOCYTES
DUAL REGULATION OF 21-HYDROXYLASE ACTIVITY BY SEX STEROID-HORMONES IN RAT HEPATOCYTES
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DOI：
10.1016/0960-0760(95)00129-n
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发表时间：
1995-08-01
影响因子：
4.1
通讯作者：
IGARASHI, Y
中科院分区：
文献类型：
--
作者：
ENDOH, A;NATSUME, H;IGARASHI, Y
In the rat liver, cytochrome P450 catalyzes the hydroxylation of steroid hormones. The expression and activity of some P450 isozymes are regulated by sex steroid hormones. Steroid 21-hydroxylase activity in rat liver is provided mainly by CYP2C6. We studied the regulation of 21-hydroxylase activity by sex steroid hormones in rat primary hepatocyte culture. We added estrogens (estrone, estradiol, estriol) and androgens (testosterone, dihydrotestosterone), ranging from 10(-9) to 10(-5) M) to the culture. The 21-hydroxlyase activity was stimulated by estrogens and was suppressed slightly by androgen in a dose-related manner. The results of our studies demonstrated that sex steroid hormones act differently on 21-hydroxylase activity in rat hepatocytes and, thus, support the hypothesis that the extra-adrenal production of deoxycorticosterone from circulating progesterone is increased during pregnancy by the massive presence of estrogens.
