Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates
Fluorescent amplified fragment length polymorphism analysis of Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis isolates
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DOI：
10.1128/aem.70.2.1068-1080.2004
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发表时间：
2004-02-01
影响因子：
4.4
通讯作者：
Jackson, PJ
中科院分区：
文献类型：
--
作者：
Hill, KK;Ticknor, LO;Jackson, PJ
采用荧光扩增片段长度多态性（AFLP）技术对300多株苏云金芽孢杆菌、蜡状芽孢杆菌和炭疽芽孢杆菌的DNA进行了分析。B。苏云金杆菌和B.蜡样芽胞杆菌分离株来自不同的来源和地点，包括土壤、临床分离株和引起腹泻和呕吐爆发的食品，以及来自美国典型培养物保藏中心的典型菌株和超过200种B。代表36个血清型或亚种的苏云金杆菌分离物来自美国农业部保藏中心。24个不同的B。还包括炭疽分离株。AFLP系统发育分析显示B内存在广泛的多样性。苏云金杆菌和B.与B的单态性质相比，炭疽病所有的B。炭疽菌菌株之间的亲缘关系较其他芽孢杆菌菌株更近，而B. cereus和B.苏云金杆菌菌株遍布整棵树。定义了10个不同的分支，其中许多分支包含两个B。cereus和B.苏云金杆菌分离物单个分支包含所有的B。炭疽菌分离株加上一个不寻常的B。苏云金杆菌分离物在小鼠中是致病性。相反，B.苏云金kurstaki（ATCC 33679）和其它用于制备杀虫剂的分离株，定位于B的远端。炭疽菌分离物。B的穿插。cereus和B.系统树中苏云金杆菌分离株的数量表明，用于区分这两个物种的表型性状并不能反映不同分离株的基因组含量，水平基因转移在建立这些微生物的表型中起着重要作用。B。特定亚种的苏云金杆菌分离物倾向于聚集在一起。
DNA from over 300 Bacillus thuringiensis, Bacillus cereus, and Bacillus anthracis isolates was analyzed by fluorescent amplified fragment length polymorphism (AFLP). B. thuringiensis and B. cereus isolates were from diverse sources and locations, including soil, clinical isolates and food products causing diarrheal and emetic outbreaks, and type strains from the American Type Culture Collection, and over 200 B. thuringiensis isolates representing 36 serovars or subspecies were from the U.S. Department of Agriculture collection. Twenty-four diverse B. anthracis isolates were also included. Phylogenetic analysis of AFLP data revealed extensive diversity within B. thuringiensis and B. cereus compared to the monomorphic nature of B. anthracis. All of the B. anthracis strains were more closely related to each other than to any other Bacillus isolate, while B. cereus and B. thuringiensis strains populated the entire tree. Ten distinct branches were defined, with many branches containing both B. cereus and B. thuringiensis isolates. A single branch contained all the B. anthracis isolates plus an unusual B. thuringiensis isolate that is pathogenic in mice. In contrast, B. thuringiensis subsp. kurstaki (ATCC 33679) and other isolates used to prepare insecticides mapped distal to the B. anthracis isolates. The interspersion of B. cereus and B. thuringiensis isolates within the phylogenetic tree suggests that phenotypic traits used to distinguish between these two species do not reflect the genomic content of the different isolates and that horizontal gene transfer plays an important role in establishing the phenotype of each of these microbes. B. thuringiensis isolates of a particular subspecies tended to cluster together.