MUTUAL INTERACTIONS AMONG CHOLINERGIC, NORADRENERGIC AND SEROTONERGIC NEURONS STUDIED BY IONOPHORESIS OF THESE TRANSMITTERS IN RAT BRAIN-STEM NUCLEI
MUTUAL INTERACTIONS AMONG CHOLINERGIC, NORADRENERGIC AND SEROTONERGIC NEURONS STUDIED BY IONOPHORESIS OF THESE TRANSMITTERS IN RAT BRAIN-STEM NUCLEI
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DOI：
10.1016/0306-4522(93)90325-a
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发表时间：
1993-08-01
期刊：
影响因子：
3.3
通讯作者：
KAYAMA, Y
中科院分区：
文献类型：
--
作者：
KOYAMA, Y;KAYAMA, Y
在麻醉大鼠，单个神经元的活动记录在或周围的中央灰质的中脑尾侧脑桥与多管微电极的乙酰胆碱，去甲肾上腺素和5-羟色胺的离子电泳应用。神经元根据锋电位的形状分为宽锋电位和短锋电位。在背侧被盖核，蓝斑或中缝背侧，宽峰神经元，标记的Pontamine天蓝色和歧视的部分处理的组织化学还原烟酰胺腺嘌呤二核苷酸磷酸黄递酶或Nissl染色，被认为是胆碱能，去甲肾上腺素能或肾上腺素能，分别。这些神经元大多数通过自身感受器被抑制，除了一些背外侧被盖神经元可能不提供的自身感受器。去甲肾上腺素和5-羟色胺抑制超过三分之二的侧背被盖神经元测试，而少数神经元兴奋去甲肾上腺素。去甲肾上腺素对中缝背核神经元的作用和5-羟色胺对蓝斑神经元的作用在许多被测神经元中尚不清楚，但这些实验中受影响的神经元（30%）均受到明显的抑制，未观察到兴奋作用。乙酰胆碱对约一半的中缝背核神经元有抑制作用，而对蓝斑神经元的作用是本实验中唯一以兴奋作用为主的，但有一半以上的蓝斑神经元对乙酰胆碱不敏感，因此，本实验获得了有关背外侧被盖胆碱能神经元药理学性质的新资料。此外，脑干胆碱能神经元，去甲肾上腺素能神经元和多巴胺能神经元之间的相互作用进行了测定，通过比较这些神经元的药理学特性测试与一个统一的程序。这些弥漫性投射神经元之间的相互作用可能涉及控制警觉、觉醒和/或睡眠的神经机制。
In urethane-anesthetized rats, single neuronal activity was recorded in or around the central gray of the caudal mesencephalon to rostral pons with multibarrel microelectrodes for ionophoretic application of acetylcholine, noradrenaline and serotonin. Neurons were classified by spike shape into broad-spike and brief-spike neurons. In the laterodorsal tegmental nucleus, locus coeruleus or dorsal raphe, broad-spike neurons, marked by Pontamine Sky Blue and discriminated in sections processed for histochemistry of reduced nicotinamide adenine dinucleotide phosphate diaphorase or Nissl staining, were presumed to be cholinergic, noradrenergic or serotonergic, respectively. The majority of these neurons were inhibited through autoreceptors, except some laterodorsal tegmental neurons which might not be furnished by autoreceptors. Noradrenaline and serotonin inhibited more than two-thirds of the laterodorsal tegmental neurons tested, while a few neurons were excited by noradrenaline. Though effects of noradrenaline on dorsal raphe neurons and those of serotonin on locus coeruleus neurons were not clear in many neurons tested, neurons affected in these examinations (30%) were all inhibited clearly and no excitatory effect was observed. Acetylcholine exerted inhibition on about one-half of dorsal raphe neurons, while effects of acetylcholine on locus coeruleus neurons were the only case in the present study in which excitation was the major effect, though more than a half of locus coeruleus neurons were not sensitive to this drug.Thus, in this study some new data on the pharmacological properties of the cholinergic laterodorsal tegmental neurons were obtained. In addition, mutual interactions between brainstem cholinergic, noradrenergic and serotonergic neurons were assayed by comparing the pharmacological properties of these neurons tested with a uniform procedure. The interactions between these diffuse projection neurons may be involved in neural mechanisms controling vigilance, wakefulness and/or sleep.