Deoxyribonucleic acid contamination in archival human temporal bones: a potentially significant problem.
Deoxyribonucleic acid contamination in archival human temporal bones: a potentially significant problem.
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档案人类颞骨中的脱氧核糖核酸污染：一个潜在的重大问题。
DOI：
10.1097/00129492-200209000-00030
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Merchant,SaumilN
中科院分区：
文献类型：
--
作者：
McKenna,MichaelJ;Kristiansen,ArthurG;Tropitzsch,AnkeS;Tranebjaerg,Lisbeth;Merchant,SaumilN
BackgroundDuring the past decade, there has been growing interest in the development of molecular biologic techniques that can be applied to the investigation of pathologic changes in archival human temporal bones. The impetus for the development of these techniques is in part related to the fact that the temporal bone collections represent a repository of archival material compiled over decades, which is not available from living patients.MethodsAn archival human temporal bone specimen from a male patient with the Mohr-Tranebjærg syndrome (formerly called DFN-1) and a well-characterized mutation was analyzed for the presence of the mutation by a standard method for extraction, isolation, amplification, and sequencing of deoxyribonucleic acid. The experiment was repeated four times.ResultsThe deoxyribonucleic acid sequence from three of four extractions was normal. The known mutation was easily and repeatedly demonstrated in a blood sample from the same individual. Because Mohr-Tranebjærg syndrome is X-linked, there is only one allele, and therefore there is no potential endogenous source to account for the normal sequence that was amplified. Contamination of the tissue sections by extraneous deoxyribonucleic acid presumably occurred during acquisition and processing of the temporal bone.ConclusionsContamination of archival temporal bones with exogenous deoxyribonucleic acid is a significant potential problem that must be considered in the interpretation of the results of deoxyribonucleic acid retrieved from archival sections. The authors recommend collecting blood samples from temporal bone donors in the future to ensure the availability of a reliable source of deoxyribonucleic acid.
