Analysis of leaf water relations in leaves of two olive (Olea europaea) cultivars differing in tolerance to salinity
Analysis of leaf water relations in leaves of two olive (Olea europaea) cultivars differing in tolerance to salinity
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DOI：
10.1093/treephys/17.1.13
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发表时间：
1997-01-01
期刊：
影响因子：
4
通讯作者：
Tattini, M
中科院分区：
文献类型：
--
作者：
Gucci, R;Lombardini, L;Tattini, M
一岁生的橄榄插条（Olea europaea L. cvs. Frantoio 和 Leccino）采用水培法或温室土壤中种植。将植物暴露于 NaCl 处理（0、100 和 200 mM）35 天，然后进行 30 至 34 天的盐胁迫缓解，以确定先前证明的耐盐性基因型差异是否与水关系参数有关。无论生长基质如何，暴露于高盐浓度都会导致两个品种的黎明前水势 (Psi(w))、完全膨压渗透势 (Psi(pi FT))、膨压损失点渗透势 (Psi(pi TLP)) 和相对含水量 (RWC) 降低。到缓解期结束时，Leaf Psi(w) 和 RWC 恢复到与对照相似的值。盐度对 Psi(pi) 的影响在 Leccino 比 Frantoio 出现得更早。对于 0、100 和 200 mM 盐处理的 Frantoio 植物，Psi(pi FT) 值分别为 -2.50、-2.87 和 -3.16 MPa，对于相应的 Leccino 植物，Psi(pi FT) 值分别为 -2.23、-2.87 和 -3.37 MPa。 100 mM 盐处理中的植物的 Psi(pi) 恢复完全，但 200 mM 盐处理中的植物则未完全恢复，与对照植物相比，其保持了增加的压力潜力 (Psi(p))。对盐敏感的品种 Leccino 的净溶质积累量高于 Frantoio。在两个品种的对照中，阳离子贡献了总Psi(pi FT)的39.9%至42.0%，甘露醇和葡萄糖贡献了27.1%至30.8%，其他可溶性碳水化合物贡献了3.1%至3.6%。 Na+的渗透贡献从未处理植物的0.1-2.1%分别增加到100 mM和200 mM盐处理植物的8.6-15.5%和15.6-20.0%。甘露醇对Psi pi(FT)的贡献在盐化期结束时达到最大值9.1%。我们得出的结论是，两个品种叶水关系之间的差异反映了 Na+ 和 Cl- 离子排斥能力的差异。
One-year-old rooted cuttings of olive (Olea europaea L. cvs. Frantoio and Leccino) were grown either hydroponically or in soil in a greenhouse. Plants were exposed to NaCl treatments (0, 100, and 200 mM) for 35 days, followed by 30 to 34 days of relief from salt stress to determine whether previously demonstrated genotypic differences in tolerance to salinity were related to water relations parameters. Exposure to high salt concentrations resulted in reductions in predawn water potential (Psi(w)), osmotic potential at full turgor (Psi(pi FT)), osmotic potential at turgor loss point (Psi(pi TLP)), and relative water content (RWC) in both cultivars, regardless of the growth substrate. Leaf Psi(w) and RWC returned to values similar to those of controls by the end of the relief period. The effect of salinity on Psi(pi) appeared earlier in Leccino than in Frantoio. Values for Psi(pi FT) were -2.50, -2.87, and -3.16 MPa for the 0, 100, and 200 mM salt-treated Frantoio plants, respectively, and -2.23, -2.87, and -3.37 MPa for the corresponding Leccino plants. Recovery of Psi(pi) was complete for plants in the 100 mM salt treatment, but not for plants in the 200 mM salt treatment, which maintained an increased pressure potential (Psi(p)) compared to control plants. Net solute accumulation was higher in Leccino, the salt-sensitive cultivar, than in Frantoio. In controls of both cultivars, cations contributed 39.9 to 42.0% of the total Psi(pi FT), mannitol and glucose contributed 27.1 to 30.8%, and other soluble carbohydrates contributed 3.1 to 3.6%. The osmotic contribution of Na+ increased from 0.1-2.1% for nontreated plants to 8.6-15.5% and 15.6-20.0% for the 100 mM and 200 mM salt-treated plants, respectively. The mannitol contribution to Psi pi(FT) reached a maximum of 9.1% at the end of the salinization period. We conclude that differences between the two cultivars in leaf water relations reflect differences in the exclusion capacities for Na+ and Cl- ions.