Genetic and molecular analysis of fox-1, a numerator element involved in Caenorhabditis elegans primary sex determination.
Genetic and molecular analysis of fox-1, a numerator element involved in Caenorhabditis elegans primary sex determination.
复制标题
Fox-1（一种参与秀丽隐杆线虫初级性别决定的分子元件）的遗传和分子分析。
作者：
Magdalena Skipper;Catherine A. Milne;Jonathan Hodgkin
Fox-1先前基于其过表达表型被鉴定为候选分子元件。FOX-1是一种RRM型RNA结合蛋白，在体外可与RNA结合。Western分析在整个发育过程中检测FOX-1。fox-1：：lacZ在胚胎发生早期普遍存在。胚胎后，fox-1：：lacZ在头部和尾部的细胞亚群中表达性别特异性。我们描述了一个Tc 1衍生的删除等位基因[福克斯-1（德尔塔）]，删除RRM域。fox-1（Delta）在XX中不赋予表型，但可以挽救XO特异性致死性和由X左端重复引起的雌性化。fox-1（Delta）与推定分子协同作用，导致XX发育异常。遗传分析表明，fox-1（Delta）导致xol-1活性略有增加，而fox-1（gf）导致xol-1活性部分丧失，xol-1对fox-1具有上位性。核糖核酸酶保护实验揭示了在fox-1（Delta）动物中2.2-kb xol-1信息的水平增加，而在fox-1（gf）动物中水平降低。此外，狐狸-1（Delta）损害男性交配效率，我们认为这代表了狐狸-1的另一种功能，独立于xol-1及其在性别决定中的作用。
fox-1 was previously identified as a candidate numerator element based on its overexpression phenotype. FOX-1 is an RRM-type RNA-binding protein, which can bind RNAs in vitro. Western analysis detects FOX-1 throughout development. fox-1::lacZ comes on ubiquitously early during embryogenesis. Postembryonically, fox-1::lacZ is expressed sex specifically in a subset of cells in the head and tail. We describe a Tc1-derived deletion allele [fox-1(Delta)] that removes the RRM domain. fox-1(Delta) confers no phenotype in XXs, but can rescue XO-specific lethality and feminization caused by duplications of the left end of the X. fox-1(Delta) synergizes with putative numerators, resulting in abnormal XX development. Genetic analysis indicated that fox-1(Delta) leads to a slight increase in xol-1 activity, while fox-1(gf) leads to partial loss of xol-1 activity, and xol-1 is epistatic to fox-1. RNase protection experiments revealed increased levels of the 2.2-kb xol-1 message in fox-1(Delta) animals, and reduced levels in fox-1(gf) animals. Additionally, fox-1(Delta) impairs male mating efficiency, which, we propose, represents another function of fox-1, independent of xol-1 and its role in sex determination.
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DOI：
10.1073/pnas.85.23.8998
发表时间：
1988-12-01
影响因子：
11.1
作者：
FROHMAN, MA;DUSH, MK;MARTIN, GR
通讯作者：
MARTIN, GR
秀丽隐杆线虫性别决定基因 Her-1 的新型显性转化等位基因。
影响因子：
3.3
作者：
Trent,C;Wood,WB;Horvitz,HR
通讯作者：
Horvitz,HR
影响因子：
3.5
作者：
HO, SN;HUNT, HD;PEASE, LR
通讯作者：
PEASE, LR
鉴定秀丽隐杆线虫中含有性别决定信号元件的 X 染色体区域。
影响因子：
3.3
作者：
Akerib,CC;Meyer,BJ
通讯作者：
Meyer,BJ
有证据表明，sisterless-a 和sisterless-b 是果蝇X/A 性别决定信号的几个离散“分子元件”中的两个，可在两种替代稳定表达状态之间切换Sxl。
影响因子：
3.3
作者：
Cline,TW
通讯作者：
Cline,TW