CLONING OF THE Y1 LOCUS OF MAIZE, A GENE INVOLVED IN THE BIOSYNTHESIS OF CAROTENOIDS
CLONING OF THE Y1 LOCUS OF MAIZE, A GENE INVOLVED IN THE BIOSYNTHESIS OF CAROTENOIDS
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DOI：
10.1105/tpc.2.9.867
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发表时间：
1990-09-01
期刊：
影响因子：
11.6
通讯作者：
ROBERTSON, DS
中科院分区：
文献类型：
--
作者：
BUCKNER, B;KELSON, TL;ROBERTSON, DS
y1基因是负责产生β的基因之一。玉米胚乳和叶子中的胡萝卜素。我们用Mu 3元件作为杂交探针克隆了y1基因的一个罗伯逊突变体标记的等位基因（y1-mum）。我们证实，克隆的序列是一个假定的突变体诱导的y1等位基因的回复突变体的分子分析的一部分，并从几个独立衍生的突变体诱导的y1突变体股票的克隆的y1序列内的插入的发生率的y1基因。y1-mum序列用于分离标准Y1等位基因，其调节β-mum的存在。玉米粒胚乳中的胡萝卜素。
The y1 gene is one of the genes responsible for the production of .beta.-carotene in the endosperm and leaves of maize. We have cloned a Robertson''s Mutator-tagged allele of the y1 gene (y1-mum) by using a Mu3 element as a hybridization probe. We substantiate that the cloned sequence is a portion of the y1 gene by molecular analyses of a revertant of a putative Mutator-induced y1 allele and the incidence of insertions within the cloned y1 sequence from several independently derived Mutator-induced y1 mutant stocks. The y1-mum sequence was used to isolated the standard Y1 allele, which conditions the presence of .beta.-carotene in the endosperm of the maize kernel.