Perturbation of the Monocyte Compartment in Human Obesity
Perturbation of the Monocyte Compartment in Human Obesity
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DOI：
10.3389/fimmu.2019.01874
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发表时间：
2019-08-08
影响因子：
7.3
通讯作者：
Rossol, Manuela
中科院分区：
文献类型：
--
作者：
Friedrich, Kathleen;Sommer, Miriam;Rossol, Manuela
循环单核细胞可分为经典单核细胞（CM）、中间单核细胞（IM）和非经典单核细胞（NCM），经典单核细胞还包含CD 56+单核细胞和单核细胞骨髓源性抑制细胞（M-MDSC）。该研究的目的是评估单核细胞亚群在人类肥胖中的发生。27名正常人、23名超重者和60名肥胖者（包括17名糖耐量正常的肥胖者和27名2型糖尿病患者）被纳入本研究。从人血液中分离外周血单核细胞，并通过流式细胞术分析鉴定单核细胞亚群的表面标记物。肥胖个体的总单核细胞、CM、IM、CD 56+单核细胞和M-MDSC数量较高。CM、IM、CD 56+单核细胞和M-MDSC的数量与体重指数、体脂、腰围、甘油三酯、C反应蛋白和HbA 1c呈正相关，与高密度脂蛋白胆固醇呈负相关。肥胖和2型糖尿病患者的IM、NCM和M-MDSC数量较高，而肥胖和糖耐量受损患者的CD 56+单核细胞数量较高。总之，对人类肥胖症患者血液单核细胞的综合分析揭示了单核细胞区室向促炎单核细胞和免疫抑制单核细胞的转变，促炎单核细胞可能有助于肥胖症患者低度炎症的发展，免疫抑制单核细胞可能有助于肥胖症患者癌症的发展。
Circulating monocytes can be divided into classical (CM), intermediate (IM), and non-classical monocytes (NCM), and the classical monocytes also contain CD56+ monocytes and monocytic myeloid-derived suppressor cells (M-MDSC). The aim of the study was to evaluate the occurrence of the monocyte subpopulations in human obesity. Twenty-seven normal, 23 overweight, and 60 obese individuals (including 17 obese individuals with normal glucose tolerance and 27 with type 2 diabetes) were included into this study. Peripheral blood mononuclear cells were isolated from human blood, and surface markers to identify monocyte subpopulations were analyzed by flow cytometry. Obese individuals had higher numbers of total monocytes, CM, IM, CD56+ monocytes, and M-MDSCs. The number of CM, IM, CD56+ monocytes, and M-MDSCs, correlated positively with body mass index, body fat, waist circumference, triglycerides, C-reactive protein, and HbA1c, and negatively with high-density lipoprotein cholesterol. Individuals with obesity and type 2 diabetes had higher numbers of IM, NCM, and M-MDSCs, whereas those with obesity and impaired glucose tolerance had higher numbers of CD56+ monocytes. In summary, the comprehensive analysis of blood monocytes in human obesity revealed a shift of the monocyte compartment toward pro-inflammatory monocytes which might contribute to the development of low-grade inflammation in obesity, and immune-suppressive monocytes which might contribute to the development of cancer in obesity.