Complete nucleotide sequences of the coat protein messenger RNAs of brome mosaic virus and cowpea chlorotic mottle virus.
Complete nucleotide sequences of the coat protein messenger RNAs of brome mosaic virus and cowpea chlorotic mottle virus.
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DOI：
10.1093/nar/10.2.703
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发表时间：
1982-01
影响因子：
14.9
通讯作者：
R. Dasgupta;P. Kaesberg
中科院分区：
文献类型：
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作者：
R. Dasgupta;P. Kaesberg
用RNA和cDNA直接测序法测定了雀麦花叶病毒（BMV）和豇豆褪绿斑驳病毒（CCMV）的亚基因组外壳蛋白信使（RNA4）的核苷酸序列。BMV RNA 4全长876 B，5'端非编码区9个核苷酸，3'端非编码区300个核苷酸。CCMVRNA 4全长824 B，包括10个核苷酸的5'端非编码区和244个核苷酸的3'端非编码区。编码的外壳蛋白长度相似（BMV为188个氨基酸，CCMV为189个氨基酸），氨基酸序列同源性约为70%。两种RNA之间的长度差异主要是由于在CCMV中相对于BMV中紧接在编码区之后的约57 B的单个缺失。允许这种缺失的RNA表明，导致分歧的突变在编码区主要受到维持有利的外壳蛋白结构的要求的限制，在3'非编码区主要受到维持有利的RNA空间构型的要求的限制。
The nucleotide sequences of the subgenomic coat protein messengers (RNA4's) of two related bromoviruses, brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV), have been determined by direct RNA and CDNA sequencing without cloning. BMV RNA4 is 876 b long including a 5' noncoding region of nine nucleotides and a 3' noncoding region of 300 nucleotides. CCMV RNA 4 is 824 b long, including a 5' noncoding region of 10 nucleotides and a 3' noncoding region of 244 nucleotides. The encoded coat proteins are similar in length (188 amino acids for BMV and 189 amino acids for CCMV) and display about 70% homology in their amino acid sequences. Length difference between the two RNAs is due mostly to a single deletion, in CCMV with respect to BMV, of about 57 b immediately following the coding region. Allowing for this deletion the RNAs are indicate that mutations leading to divergence were constrained in the coding region primarily by the requirement of maintaining a favorable coat protein structure and in the 3' noncoding region primarily by the requirement of maintaining a favorable RNA spatial configuration.