Platelet‐derived growth factor promotes the proliferation of human umbilical cord‐derived mesenchymal stem cells
Platelet‐derived growth factor promotes the proliferation of human umbilical cord‐derived mesenchymal stem cells
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DOI：
10.1002/cbf.2870
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发表时间：
2013-03
影响因子：
3.6
通讯作者：
P. Qiu;Wencong Song;Z. Niu;Yaofu Bai;Wei Li;S. Pan;S. Peng;J. Hua
中科院分区：
文献类型：
--
作者：
P. Qiu;Wencong Song;Z. Niu;Yaofu Bai;Wei Li;S. Pan;S. Peng;J. Hua
本研究旨在观察血小板衍生生长因子（PDGF）对人脐带间充质干细胞（UC-MSCs）增殖的影响，并进一步探讨PDGF促进UC-MSCs增殖的机制。以不同浓度的PDGF处理人UC-MSCs，通过细胞计数、细胞活力、RT-PCR分析PDGF受体表达、免疫荧光染色和真实的时间定量（QRT-PCR）检测细胞增殖、细胞周期和多能性基因表达、Brdu测定等方法评价其作用效果。结果显示，PDGF对体外培养的UC-MSCs有促进增殖作用，且呈剂量依赖性，10 ~ 50 ng/ml的PDGF对UC-MSCs有明显的增殖作用，其中以50 ng/ml的作用最为明显。当PDGF浓度高于100 ng/ml时，观察到UC-MSCs的增殖受到显著抑制，当PDGF浓度达到200 ng/ml时，所有细胞死亡。Brdu染色显示PDGF处理组细胞的增殖能力和抗凋亡能力均强于对照组。与对照组相比，PDGF培养液中增殖相关基因C-MYC、PCNA和TERT以及细胞周期相关基因cyclin A、cyclin 1和CDK 2的表达上调。然而，在PDGF中培养的细胞与通过免疫荧光和QRT-PCR分析的细胞之间，多能基因OCT 4没有显著差异。PDGF能促进人UC‐MSCs的体外增殖。版权所有© 2012约翰威利父子有限公司.
This study was designed to investigate the effect of platelet‐derived growth factor (PDGF) on the proliferation of human umbilical cord mesenchymal stem cells (UC‐MSCs) and further explore the mechanism of PDGF in promoting the proliferation of UC‐MSCs. The human UC‐MSCs were treated with different concentrations of PDGF, and the effects were evaluated by counting the cell number, the cell viability, the expression of PDGF receptors analyzed by RT‐PCR, and the detection of the gene expression of cell proliferation, cell cycle and pluripotency, and Brdu assay by immunofluorescent staining and Quantitative real‐time (QRT‐PCR). The results showed that PDGF could promote the proliferation of UC‐MSCs in vitro in a dose‐dependent way, and 10 to 50 ng/ml PDGF had a significant proliferation effect on UC‐MSCs; the most obvious concentration was 50 ng/ml. Significant inhibition on the proliferation of UC‐MSCs was observed when the concentration of PDGF was higher than 100 ng/ml, and all cells died when the concentration reached 200 ng/ml PDGF. The PDGF‐treated cells had stronger proliferation and antiapoptotic capacity than the control group by Brdu staining. The expression of the proliferation‐related genes C‐MYC, PCNA and TERT and cell cycle–related genes cyclin A, cyclin 1 and CDK2 were up‐regulated in PDGF medium compared with control. However, pluripotent gene OCT4 was not significantly different between cells cultured in PDGF and cells analyzed by immunofluorescence and QRT‐PCR. The PDGF could promote the proliferation of human UC‐MSCs in vitro. Copyright © 2012 John Wiley & Sons, Ltd.