Facile fabrication of uniform size-controlled microparticles and potentiality for tandem drug delivery system of micro/nanoparticles
Facile fabrication of uniform size-controlled microparticles and potentiality for tandem drug delivery system of micro/nanoparticles
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DOI：
10.1016/j.colsurfb.2013.04.007
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发表时间：
2013-09-01
影响因子：
5.8
通讯作者：
Nakamura, Makoto
中科院分区：
文献类型：
--
作者：
Iwanaga, Shintaroh;Saito, Noriaki;Nakamura, Makoto
本文介绍了一种利用喷墨打印技术快速简便地制造各种尺寸可控的凝胶颗粒的方法。一般来说，通过改变喷墨打印机的喷嘴头来控制液滴的大小，从喷嘴中喷射出油墨溶液。然而，该方法在微颗粒胶凝前采用干燥工艺，只需改变喷射溶液的浓度即可轻松控制微颗粒的大小。当从喷墨打印机中喷射不同浓度的海藻酸钠溶液时，我们发现在0.1 ~ 3.0%的浓度范围内，海藻酸钠溶液的浓度与干燥的海藻酸钠颗粒的体积几乎呈线性关系。将干燥后的海藻酸盐颗粒浸泡在氯化钙溶液中，得到的微凝胶珠的大小几乎没有增加。通过喷射纳米颗粒分散的海藻酸盐溶液，可以很容易地制备出各种尺寸的纳米颗粒。海藻酸盐微凝胶珠中25纳米颗粒的释放速度相对较快，而100纳米颗粒的部分释放速度较快。此外，海藻酸盐微凝胶珠浸泡24 h后，大部分250 nm大小的纳米颗粒仍未从微凝胶珠中释放出来。该方法可以实现纳米颗粒和微颗粒释放相结合的串联给药系统，有望在生物和组织工程领域得到应用。(C) 2013 Elsevier B.V.版权所有
This article describes a rapid and facile method for manufacturing various size-controlled gel particles with utilizing inkjet printing technology. Generally, the size of droplets could be controlled by changing nozzle heads of inkjet printer, from which ink solution is ejected. However, this method uses drying process before gelling microparticles, and with that, the size of microparticles was easily controlled by only altering the concentration of ejected solution. When sodium alginate solution with various concentrations was ejected from inkjet printer, we found that the concentration of alginate solution vs. the volume of dried alginate particle showed an almost linear relationship in the concentration range from 0.1 to 3.0%. After dried alginate particles were soaked into calcium chloride solution, the size of microgel beads were obtained almost without increasing their size. The microparticles including various sizes of nanoparticles were easily manufactured by ejecting nanoparticle-dispersed alginate solution. The release of 25-nm sized nanoparticles from alginate microgel beads was finished in a relatively-rapid manner, whereas 100-nm sized nanoparticles were partially released from those ones. Moreover, most of 250-nm sized nanoparticles were not released from alginate microgel beads even after 24-h soaking. This particle fabricating method would enable the tandem drug delivery system with a combination of the release from nano and microparticles, and be expected for the biological and tissue engineering application. (C) 2013 Elsevier B.V. All rights reserved.