LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04
LncRNA FEZF1-AS1 Promotes TGF-β2-Mediated Proliferation and Migration in Human Lens Epithelial Cells SRA01/04
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DOI：
10.1155/2019/4736203
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发表时间：
2019-01-01
影响因子：
1.9
通讯作者：
Gu, Shuyan
中科院分区：
文献类型：
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作者：
Wang, Yong;Chen, Lili;Gu, Shuyan
后囊混浊是白内障手术后常见的并发症，其原因是术后残留的透镜上皮细胞（LEC）增殖和迁移。长链非编码RNA（lncRNA）FEZ家族锌指1反义RNA 1（FEZF 1-AS 1）促进多种类型癌细胞的增殖和迁移。在此，我们发现FEZF 1-AS 1在TGF-2处理的SRA 01/04细胞中显著上调。TGF-2处理后，SRA 01/04细胞的增殖和迁移能力增强。FEZF 1-AS 1基因敲低可抑制TGF-2诱导的SRA 01/04细胞增殖和迁移。因此，FEZF 1-AS 1过表达促进了TGF-2诱导的SRA 01/04细胞增殖和迁移。FEZF 1-AS 1通过上调FEZF 1蛋白水平，上调TGF-2诱导的SRA 01/04细胞增殖和迁移。我们的研究结果表明，FEZF 1-AS 1的失调参与TGF-2诱导的人透镜上皮细胞（HLEC）的增殖和迁移，这可能是实现的，至少部分，通过诱导FEZF 1的表达。
Posterior capsule opacification (PCO) is a common complication after cataract surgery attributed to the proliferation and migration of postoperative residual lens epithelial cells (LECs). The long noncoding RNA (lncRNA) FEZ family zinc finger 1 antisense RNA 1 (FEZF1-AS1) promotes the proliferation and migration of multiple types of cancer cells. Here, we discovered that FEZF1-AS1 is markedly upregulated in TGF-2-treated SRA01/04cells. In addition, the proliferation and migration of SRA01/04cells were enhanced following TGF-2 treatment. FEZF1-AS1 knockdown inhibited the TGF-2-induced proliferation and migration of SRA01/04cells. Accordingly, FEZF1-AS1 overexpression promoted the TGF-2-induced proliferation and migration of SRA01/04cells. Finally, FEZF1-AS1 upregulated TGF-2-induced SRA01/04cell proliferation and migration via boosting FEZF1 protein levels. Our findings indicate that the dysregulation of FEZF1-AS1 participates in the TGF-2-induced proliferation and migration of human lens epithelial cells (HLECs), which might be achieved, at least in part, through the induction of FEZF1 expression.