Allelic Variation of MYB10 Is the Major Force Controlling Natural Variation in Skin and Flesh Color in Strawberry (Fragaria spp.) Fruit
Allelic Variation of MYB10 Is the Major Force Controlling Natural Variation in Skin and Flesh Color in Strawberry (Fragaria spp.) Fruit
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DOI：
10.1105/tpc.20.00474
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发表时间：
2020-12-01
期刊：
影响因子：
11.6
通讯作者：
Amaya, Iraida
中科院分区：
文献类型：
--
作者：
Castillejo, Cristina;Waurich, Veronika;Amaya, Iraida
二倍体野生型草莓（Fragaria vesca）和二倍体栽培型草莓（Fragaria x ananassa）的花色苷变异主要由转录因子MYB 10的独立突变引起，而二倍体和二倍体草莓（Fragaria spp）的花色苷积累和分布模式不同，导致果实颜色的自然变异。花青苷的合成受一个R2 R3 MYB转录因子控制，其中MYB 10是草莓果实中的主要激活因子。在这里，我们发现MYB 10的突变导致了在二倍体林地草莓（F。vesca）和四倍体栽培草莓（F. xananassa）。利用测序作图的方法，我们在MYB 10中鉴定了一个gypsy转座子，该转座子可以截短蛋白质并敲除白果F. vesca生态型另外两个MYB 10的功能缺失突变在地理上不同的白果F。vesca生态型四倍体草莓属植物的遗传和转录组学分析表明，FaMYB 10 -2，三个MYB 10同源物之一，确定，调控花青素的生物合成在发展中的水果。此外，MYB 10 -2的独立突变是四倍体草莓果皮和果肉颜色自然变异的根本原因。我们确定了一个CACTA样转座子（FaEnSpm-2）插入MYB 10 -2启动子的红肉加入，这是与增强表达。我们的研究结果表明，FaEnSpm-2中的顺式调控元件负责增强草莓果肉中MYB 10 -2的表达和花青素的生物合成。
Independent mutations in the transcription factor MYB10 cause most of the anthocyanin variation observed in diploid woodland strawberry (Fragaria vesca) and octoploid cultivated strawberry (Fragaria x ananassa).The fruits of diploid and octoploid strawberry (Fragaria spp) show substantial natural variation in color due to distinct anthocyanin accumulation and distribution patterns. Anthocyanin biosynthesis is controlled by a clade of R2R3 MYB transcription factors, among which MYB10 is the main activator in strawberry fruit. Here, we show that mutations in MYB10 cause most of the variation in anthocyanin accumulation and distribution observed in diploid woodland strawberry (F. vesca) and octoploid cultivated strawberry (F. xananassa). Using a mapping-by-sequencing approach, we identified a gypsy-transposon in MYB10 that truncates the protein and knocks out anthocyanin biosynthesis in a white-fruited F. vesca ecotype. Two additional loss-of-function mutations in MYB10 were identified among geographically diverse white-fruited F. vesca ecotypes. Genetic and transcriptomic analyses of octoploid Fragaria spp revealed that FaMYB10-2, one of three MYB10 homoeologs identified, regulates anthocyanin biosynthesis in developing fruit. Furthermore, independent mutations in MYB10-2 are the underlying cause of natural variation in fruit skin and flesh color in octoploid strawberry. We identified a CACTA-like transposon (FaEnSpm-2) insertion in the MYB10-2 promoter of red-fleshed accessions that was associated with enhanced expression. Our findings suggest that cis-regulatory elements in FaEnSpm-2 are responsible for enhanced MYB10-2 expression and anthocyanin biosynthesis in strawberry fruit flesh.