FUNGAL ELICITOR-MEDIATED RESPONSES IN PINE CELL-CULTURES .2. CELL WALL-BOUND PHENOLICS
FUNGAL ELICITOR-MEDIATED RESPONSES IN PINE CELL-CULTURES .2. CELL WALL-BOUND PHENOLICS
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DOI：
10.1016/0031-9422(92)80004-x
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发表时间：
1992-03-01
期刊：
影响因子：
3.8
通讯作者：
ELLIS, BE
中科院分区：
文献类型：
--
作者：
CAMPBELL, MM;ELLIS, BE
通过皂化、溶剂萃取、凝胶渗透色谱、[C-14]苯丙氨酸补料、苯丙氨酸解氨酶抑制和碱性硝基苯氧化产物分析等方法，对培养的班克松细胞中积累的细胞壁结合物质进行了表征。 这些分析表明，从引发细胞回收的壁结合材料具有真正的裸子植物木质素的特征，并且引发细胞中积累的木质素的量显著大于对照培养物。 细胞壁结合的阿魏酸酯和醚的含量也提高了在引发的文化。
The cell wall-bound material which accumulated in cultured Pinus banksiana cells after treatment with an elicitor derived from an ectomycorrhizal fungus was characterized by a combination of saponification, solvent extraction, gel permeation chromatography, [C-14]phenylalanine feeding, phenylalanine ammonia-lyase inhibition, and analysis of alkaline nitrobenzene oxidation products. These analyses revealed that the wall-bound material recovered from elicited cells had the features of true gymnosperm lignin and that a significantly greater amount of lignin accumulated in elicited cells than in control cultures. The contents of cell wall-bound esters and ethers of ferulic acid were also enhanced in the elicited cultures.