Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean
Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean
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基于植物激素相关基因转录组分析的大豆乙烯调控网络构建及转录因子活性预测
DOI：
10.1007/s11738-012-1170-0
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发表时间：
2013-04-01
影响因子：
2.6
通讯作者：
Liu, Chunming
中科院分区：
文献类型：
--
作者：
Cheng, Yunqing;Liu, Jianfeng;Liu, Chunming
大豆（Glycine maxL.）导致田间栽培条件下产量损失严重。乙烯是一种调节大豆发育变化和开花率的植物激素。很明显，不同的植物激素影响重叠的生理过程，植物激素的生理效应取决于特定的激素组合，而不是每个独立的活动。关于乙烯是如何整合到植物激素代谢和信号网络中的知之甚少。硫代硫酸银（STS）、乙烯利（ETH）和对照处理后的植物激素含量分析和3个RNA-seq文库结果表明，STS处理通过抑制1-氨基环丙烷-1-羧酸（ACC）合成基因的mRNA丰度来影响大豆的乙烯生物合成和信号通路，而ETH处理则同时诱导了乙烯生物合成和信号通路组分。与IAA、GA、CTK和阿坝途径相关的多个基因被激活或抑制。通过对差异转录组中转录因子的分析以及对所有激素生物合成相关的差异转录基因的预测，表明转录因子MYB在乙烯与整个激素代谢和信号网络相互作用的过程中起着关键作用。
High floral abscission ratio in soybean (Glycine maxL.) leads to serious yield loss in field culture condition. Ethylene is a phytohormone responsible for the regulation of developmental changes and floral abscission ratio in soybean. It is clear that different phytohormones affected overlapping physiological processes, and the physiological effects of phytohormone depended on specific hormone combination rather than the independent activity of each one. Little is known about how ethylene is integrated into the phytohormone metabolism and signal network. The results of phytohormone content analysis and three RNA-seq libraries after silver thiosulfate (STS), ethephon (ETH) and control treatment showed that ethylene biosynthesis and signal pathway was affected by STS treatment according to suppress the mRNA abundance of 1-aminocyclopropane-1-carboxylate (ACC) syntheses genes, while the ETH treatment induced both ethylene biosynthesis and signal pathway components in soybean. A number of genes involved in IAA, GA, CTK and ABA pathways components were activated or depressed correspondingly. The analysis of transcription factors (TFs) in differential transcriptome profiling and TFs prediction of the differentially transcription genes related to all phytohormone biosynthesis showed that TFs MYB played pivotal roles in the process of ethylene interplaying the whole phytohormones metabolism and signal network.