Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
Identification of immunogenic proteins of the bacterium Acinetobacter baumannii using a proteomic approach
复制标题
DOI：
10.1002/prca.201300133
复制
发表时间：
2014-12-01
影响因子：
2
通讯作者：
Moreno Senna, Jose Procopio
中科院分区：
文献类型：
--
作者：
Bonin, Renata Fajardo;Chapeaurouge, Alex;Moreno Senna, Jose Procopio
目的鲍曼不动杆菌是引起免疫功能低下患者肺炎、尿路感染和/或败血症的重要条件致病菌。这种病原体经常与世界范围内的医院暴发有关，并且由于其流行和对几种抗生素的耐药性模式而变得特别成问题。在本研究中，我们使用基于免疫蛋白质组的方法来鉴定鲍曼不动杆菌表面的免疫原性蛋白，以开发针对这种破坏性细菌感染的可能的免疫疗法。实验设计采用Western blot分析鲍曼不动杆菌感染患者（n = 50）和健康对照组（n = 3）的血清对鲍曼不动杆菌外膜蛋白（OMPs）的反应性。为了鉴定鲍曼不动杆菌中潜在的免疫原性蛋白，采用2DE分离omp，随机选取感染患者的反应性血清分为两个不同的池，每个池含有15份血清。最后采用MALDI-TOF/TOF质谱分析鉴定相应的蛋白。结果共鉴定出6种免疫反应蛋白：OmpA、Omp34kDa、OprC、oprb样蛋白、OXA-23和铁铁受体蛋白。值得注意的是，这些蛋白质在细菌表面非常丰富，并与毒力、抗生素耐药性和生长有关。结论和临床意义本研究结果支持免疫蛋白质组研究中鉴定的蛋白可作为开发鲍曼不动杆菌感染疫苗和被动免疫疗法的抗原候选物。
PurposeAcinetobacter baumannii is an important opportunistic pathogen that causes pneumoniae, urinary tract infections, and/or septicemia in immunocompromised patients. This pathogen is frequently associated with nosocomial outbreaks worldwide and has become particularly problematic because of its prevalence and resistance patterns to several antibiotics. In the present study, we used an immunoproteome-based approach to identify immunogenic proteins located on the surface of A. baumannii for the development of a possible immunotherapy against this devastating bacterial infection.Experimental designSera from patients with A. baumannii infections (n = 50) and from a control group of healthy individuals (n = 3) were analyzed for reactivity against A. baumannii outer membrane proteins (OMPs) using Western blot analysis. To identify potential immunogenic proteins in A. baumannii, OMPs were separated by 2DE, and reactive sera from infected patients were randomly selected and divided into two different pools, each containing 15 sera. Finally, MALDI-TOF/TOF mass spectrometric analysis was employed to identify the corresponding proteins.ResultsThis analysis identified six immunoreactive proteins: OmpA, Omp34kDa, OprC, OprB-like, OXA-23, and ferric siderophore receptor protein. Notably, these proteins are highly abundant on the bacterial surface and involved in virulence, antibiotic resistance, and growth.Conclusions and clinical relevanceOur results support the notion that the proteins identified in the present immunoproteome study could serve as antigen candidates for the development of vaccines and passive immunotherapies against A. baumannii infections.