Transcript profile analysis reveals important roles of jasmonic acid signalling pathway in the response of sweet potato to salt stress.
Transcript profile analysis reveals important roles of jasmonic acid signalling pathway in the response of sweet potato to salt stress.
复制标题
转录谱分析揭示茉莉酸信号通路在甘薯盐胁迫响应中的重要作用
DOI：
10.1038/srep40819
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发表时间：
2017-01-13
影响因子：
4.6
通讯作者：
He S
中科院分区：
文献类型：
--
作者：
Zhang H;Zhang Q;Zhai H;Li Y;Wang X;Liu Q;He S
甘薯是一种重要的粮食作物和生物能源作物，研究甘薯的耐盐机制将为甘薯的耐盐育种提供依据。本研究比较了盐敏感品种梨子香和耐盐品系ND98的根转录产物，以确定参与盐胁迫反应的基因和途径。总共有8,744个和10,413个差异表达基因(Deg)分别在梨子香和ND98中参与盐分反应。ND98的DNA甲基化水平低于荔子香。在这两种类型中，参与植物激素合成和信号传递以及离子稳态的degs可能是不同耐盐性的基础。与梨子香相比，ND98茉莉酸(JA)生物合成、信号转导和离子转运相关基因显著上调，茉莉酸积累量增加，气孔关闭程度提高，Na+含量降低。这是关于甘薯耐盐性转录组反应的首次报道。这些结果表明，JA信号通路在甘薯对盐胁迫的反应中起着重要作用。这项研究为甘薯耐盐性的机制和基因提供了深入的了解。
Sweet potato is an important food and bio-energy crop, and investigating the mechanisms underlying salt tolerance will provide information for salt-tolerant breeding of this crop. Here, the root transcriptomes of the salt-sensitive variety Lizixiang and the salt-tolerant line ND98 were compared to identify the genes and pathways involved in salt stress responses. In total, 8,744 and 10,413 differentially expressed genes (DEGs) in Lizixiang and ND98, respectively, were involved in salt responses. A lower DNA methylation level was detected in ND98 than in Lizixiang. In both genotypes, the DEGs, which function in phytohormone synthesis and signalling and ion homeostasis, may underlie the different degrees of salt tolerance. Significant up-regulations of the genes involved in the jasmonic acid (JA) biosynthesis and signalling pathways and ion transport, more accumulation of JA, a higher degree of stomatal closure and a lower level of Na+ were found in ND98 compared to Lizixiang. This is the first report on transcriptome responses to salt tolerance in sweet potato. These results reveal that the JA signalling pathway plays important roles in the response of sweet potato to salt stress. This study provides insights into the mechanisms and genes involved in the salt tolerance of sweet potato.