Functional analyses of cotton (Gossypium hirsutum L.) immature fiber (im) mutant infer that fiber cell wall development is associated with stress responses.
Functional analyses of cotton (Gossypium hirsutum L.) immature fiber (im) mutant infer that fiber cell wall development is associated with stress responses.
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DOI：
10.1186/1471-2164-14-889
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发表时间：
2013-12-17
期刊：
影响因子：
4.4
通讯作者：
Fang DD
中科院分区：
文献类型：
--
作者：
Kim HJ;Tang Y;Moon HS;Delhom CD;Fang DD
棉花纤维成熟度是决定棉花商品价值的重要因素。纤维细胞壁发育如何影响纤维成熟度还不清楚。纤维横截面的比较表明，未成熟的纤维（IM）突变体有较低的纤维成熟度比其近等基因野生型，得克萨斯州标记-1（TM-1）。im突变体和TM-1的可用性提供了一种独特的方式来确定调控棉花纤维成熟的分子机制。转录组分析表明，在正常胁迫条件下生长的im突变纤维中的差异表达基因（DEG）与在严重胁迫条件下生长的野生型棉纤维中的差异表达基因（DEG）相似。这些DEG在im突变体中的大多数与应激反应和细胞呼吸有关。已知应激降低负责能量产生和活性氧（ROS）积累的经典呼吸途径的活性。如果im突变体与应激反应相关，则im突变体纤维中的能量产生和ROS水平预计都会降低。与预测结果雅阁的是，im突变体的转录组谱显示了与能量剥夺植物中发生的转录调控相同的改变，其中与细胞生长过程相关的基因表达降低，而与回收和运输过程相关的基因表达升高。我们证实，活性氧生产在发展中的纤维从im突变体低于野生型。在im突变体纤维中较低的ROS产生可能是由于应激诱导的交替呼吸水平升高所致。非突变体纤维的纤维细胞壁厚度较低，这与参与应激反应和细胞呼吸的基因失调有关。ROS水平的降低和参与交替呼吸的基因的上调表明，能量剥夺可能发生在im突变纤维中。
Cotton fiber maturity is an important factor for determining the commercial value of cotton. How fiber cell wall development affects fiber maturity is not well understood. A comparison of fiber cross-sections showed that an immature fiber (im) mutant had lower fiber maturity than its near isogenic wild type, Texas marker-1 (TM-1). The availability of the im mutant and TM-1 provides a unique way to determine molecular mechanisms regulating cotton fiber maturity. Transcriptome analysis showed that the differentially expressed genes (DEGs) in the im mutant fibers grown under normal stress conditions were similar to those in wild type cotton fibers grown under severe stress conditions. The majority of these DEGs in the im mutant were related to stress responses and cellular respiration. Stress is known to reduce the activity of a classical respiration pathway responsible for energy production and reactive oxygen species (ROS) accumulation. Both energy productions and ROS levels in the im mutant fibers are expected to be reduced if the im mutant is associated with stress responses. In accord with the prediction, the transcriptome profiles of the im mutant showed the same alteration of transcriptional regulation that happened in energy deprived plants in which expressions of genes associated with cell growth processes were reduced whereas expressions of genes associated with recycling and transporting processes were elevated. We confirmed that ROS production in developing fibers from the im mutant was lower than that from the wild type. The lower production of ROS in the im mutant fibers might result from the elevated levels of alternative respiration induced by stress. The low degree of fiber cell wall thickness of the im mutant fibers is associated with deregulation of the genes involved in stress responses and cellular respiration. The reduction of ROS levels and up-regulation of the genes involved in alternative respirations suggest that energy deprivation may occur in the im mutant fibers.
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