Porcine model for vascular graft studies.
Porcine model for vascular graft studies.
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DOI：
10.3109/08941939109140760
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
Symes, J F
中科院分区：
文献类型：
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作者：
Mehran, R J;Ricci, M A;Symes, J F
用于研究人工血管移植物感染的模型已在狗和兔子中进行了频繁的研究。我们开发了一种可重复的猪模型来研究这个问题及其治疗方法。 The cardiovascular system, healing characteristics, and the bloodstream clearance of bacteria in swine more closely resembles those of humans than do other animal models.猪的低成本和可用性是另一个有吸引力的方面。 One hundred fifty-six farm-bred pigs have undergone infrarenal aortic replacement with a 3-cm segment of 6-mm prosthetic graft over the past two years. Graft infection was produced by (1) direct inoculation of 10(6) Staphylococcus aureus at the time of the surgery or (2) intravenous infusion of bacteria (10(2)-10(6) organisms/mL) immediately after surgery.根据研究设计，1 至 4 周后处死所有动物。对每个移植物进行培养、组织学和电子显微镜检查。麻醉并发症很少见（2.5%）。 Postoperative complications leading to animal death decreased with increasing experience (11.5%), but included graft thrombosis, bleeding, sepsis, intussusception, and colonic ischemia.伤口感染是最常见的发病原因。对于研究人工血管移植物感染，猪模型是其他动物模型的一个有吸引力的替代方案。讨论了手术技术的更多细节以及与人类和其他动物模型的比较。
Models for the study of prosthetic vascular graft infection have been studied frequently in the dog and rabbit. We have developed a reproducible swine model to study this problem and its treatment. The cardiovascular system, healing characteristics, and the bloodstream clearance of bacteria in swine more closely resembles those of humans than do other animal models. The low cost and availability of the swine is an additional attractive aspect. One hundred fifty-six farm-bred pigs have undergone infrarenal aortic replacement with a 3-cm segment of 6-mm prosthetic graft over the past two years. Graft infection was produced by (1) direct inoculation of 10(6) Staphylococcus aureus at the time of the surgery or (2) intravenous infusion of bacteria (10(2)-10(6) organisms/mL) immediately after surgery. All animals were sacrificed 1 to 4 weeks later, depending on the study design. Cultures, histology, and electron microscopy were performed on each graft. Anesthetic complications were rare (2.5%). Postoperative complications leading to animal death decreased with increasing experience (11.5%), but included graft thrombosis, bleeding, sepsis, intussusception, and colonic ischemia. Wound infection was the most common cause of morbidity. The swine model is an attractive alternative to that of other animals for the study of prosthetic vascular graft infections. Further details of the operative technique and the comparison to the human and other animal models is discussed.