THE ROLE OF ELECTROOSMOSIS IN THE ELECTRIC-FIELD-INDUCED MOVEMENT OF CHARGED MACROMOLECULES ON THE SURFACES OF CELLS
THE ROLE OF ELECTROOSMOSIS IN THE ELECTRIC-FIELD-INDUCED MOVEMENT OF CHARGED MACROMOLECULES ON THE SURFACES OF CELLS
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DOI：
10.1016/s0006-3495(81)84838-2
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发表时间：
1981-01-01
影响因子：
3.4
通讯作者：
POO, MM
中科院分区：
文献类型：
--
作者：
MCLAUGHLIN, S;POO, MM
大多数细胞的表面带有净负电荷。施加平行于电池表面的电场应产生朝向电池阴极侧的流体电渗流。对细胞表面的简单模型的分析表明，如果带负电荷的移动的大分子的ζ电位ζ 1比细胞表面的ζ电位ζ 2负得更少，则带负电荷的大分子将被流体的这种电渗流扫到细胞的阴极侧。如果ζ 2的负性小于ζ 1，则带负电荷的大分子将在电池的阳极侧积累。实验结果表明，胚胎[非洲爪蟾]肌细胞上的伴刀豆球蛋白A（Con A）受体通常在细胞的阴极侧积累，但通过用神经氨酸酶（一种去除带负电荷的唾液酸残基的处理）或用脂质二[3，3“双十八烷基吲哚羰花青碘化物]预孵育肌管，在细胞表面增加正电荷的处理。添加带负电荷的脂质单唾液酸神经节苷脂（GM 1）增强了Con A受体在细胞阴极侧的积累。
The surfaces of most cells bear a net negative charge. The imposition of an electric field parallel to the surface of the cell should produce an electro-osmotic flow of fluid towards the cathodal side of the cell. Analysis of a simple model of the cell surface indicates that a negatively charged mobile macromolecule will be swept by this electro-osmotic flow of fluid to the cathodal side of the cell if its zeta potential, .zeta.1, is less negative than the zeta potential of the cell surface, .zeta.2. If .zeta.2 is less negative than .zeta.1, the negatively charged macromolecule will accumulate at the anodal side of the cell. Experimental results demonstrate that concanavalin A (Con A) receptors on embryonic [Xenopus] muscle cells normally accumulate at the cathodal side of the cell, but that they can be induced to accumulate at the anodal side of the cell by preincubating the myotubes either with neuraminidase, a treatment that removes negatively charged sialic acid residues, or with the lipid diI [3,3''dioctadecylindocarbocyanine iodide], a treatment that adds positive charges to the surface of the cell. Addition of the negatively charged lipid monosialoganglioside (GM1) enhances the accumulation of Con A receptors at the cathodal side of the cell.