YbeY Controls the Type III and Type VI Secretion Systems and Biofilm Formation through RetS in Pseudomonas aeruginosa
YbeY Controls the Type III and Type VI Secretion Systems and Biofilm Formation through RetS in Pseudomonas aeruginosa
复制标题
YbeY通过RetS调控铜绿假单胞菌III型和VI型分泌系统及生物膜形成
DOI：
10.1128/aem.02171-20
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发表时间：
2021-03-01
影响因子：
4.4
通讯作者：
Wu, Weihui
中科院分区：
文献类型：
--
作者：
Xia, Yushan;Xu, Congjuan;Wu, Weihui
YbeY 是细菌中高度保守的 RNase，在 16S rRNA 的成熟、小 RNA (sRNA) 的调节以及细菌对环境应激的反应中发挥着重要作用。此前，我们验证了 YbeY 在铜绿假单胞菌 rRNA 加工和核糖体成熟中的作用，并证明了 YbeY 通过 sRNA ReaL 介导的 rpoS 调节。在这项研究中，我们证明ybeY基因的突变导致III型分泌系统(T3SS)基因的上调以及VI型分泌系统(T6SS)基因的下调和生物膜形成的减少。通过检查铜绿假单胞菌中已知 sRNA 的表达，我们发现 ybeY 基因的突变导致小 RNA RsmY/Z 的下调，而小 RNA RsmY/Z 控制 T3SS、T6SS 和生物膜形成。进一步的研究表明，RsmY/Z 水平降低是由于 retS 的上调。总而言之，我们的结果揭示了 YbeY 的多效性功能，并提供了 YbeY 介导的铜绿假单胞菌调节的详细机制。 重要性 铜绿假单胞菌引起人类多种急性和慢性感染。 III型分泌系统（T3SS）在急性感染中发挥重要作用，VI型分泌系统（T6SS）和生物膜形成与慢性感染相关。了解控制急性和慢性感染毒力决定因素的机制将为制定有效的治疗策略提供线索。我们的结果揭示了铜绿假单胞菌中 T3SS、T6SS 和生物膜形成的新型 RNase 介导调节。
YbeY is a highly conserved RNase in bacteria and plays essential roles in the maturation of 16S rRNA, regulation of small RNAs (sRNAs), and bacterial responses to environmental stresses. Previously, we verified the role of YbeY in rRNA processing and ribosome maturation in Pseudomonas aeruginosa and demonstrated YbeY-mediated regulation of rpoS through an sRNA, ReaL. In this study, we demonstrate that mutation of the ybeY gene results in upregulation of the type Ill secretion system (T3SS) genes as well as downregulation of the type VI secretion system (T6SS) genes and reduction of biofilm formation. By examining the expression of the known sRNAs in P. aeruginosa, we found that mutation of the ybeY gene leads to downregulation of the small RNAs RsmY/Z, which control the T3SS, T6SS, and biofilm formation. Further studies revealed that the reduced levels of RsmY/Z are due to upregulation of retS. Taken together, our results reveal the pleiotropic functions of YbeY and provide detailed mechanisms of YbeY-mediated regulation in P. aeruginosa.IMPORTANCE Pseudomonas aeruginosa causes a variety of acute and chronic infections in humans. The type III secretion system (T3SS) plays an important role in acute infection, and the type VI secretion system (T6SS) and biofilm formation are associated with chronic infections. Understanding of the mechanisms that control the virulence determinants involved in acute and chronic infections will provide clues for the development of effective treatment strategies. Our results reveal a novel RNase-mediated regulation of T3SS, T6SS, and biofilm formation in P. aeruginosa.