Triethyl tin-induced myelin oedema: an intermediate swelling state detected by X-ray diffraction.
Triethyl tin-induced myelin oedema: an intermediate swelling state detected by X-ray diffraction.
复制标题
三乙基锡诱导的髓磷脂水肿：通过 X 射线衍射检测到的中间肿胀状态。
DOI：
10.1007/bf01262424
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Sapirstein,VS
中科院分区：
文献类型：
--
作者：
Kirschner,DA;Sapirstein,VS
使用 X 射线衍射来探讨三乙基锡 (TET) 对体外呼吸视神经和坐骨神经以及通过饮用水进行体内治疗的大鼠神经的层状髓鞘膜定向障碍的周期性和数量的影响。衍射图显示，浓度为 4–100 μm 的体外 TET 会影响中枢神经系统 (C.N.S.)。但不是 P.N.S.髓磷脂结构。在中枢神经系统中检测到一个周期为 200 Å 的平面同心膜阵列；这种有序、肿胀的髓磷脂与 TET 引起的水肿薄切片中看到的空泡和囊泡结构形成鲜明对比。在中枢神经系统和中枢神经系统中均未观察到 TET 短期体内治疗的影响。或 P.N.S.碳酸酐酶 (CA) 抑制剂对 TET 诱导的结构变化没有影响的发现表明，我们观察到的膨胀与 CA 依赖性过程无关。相比之下，通过用等渗蔗糖替代移动离子可以防止 TET 效应。我们的结论是 TET 引起的中枢神经系统肿胀。髓磷脂是由离子运输的增加以及随后必然的液体运动产生的。此外，我们检测到的有序肿胀结构可能是 TET 中毒体内短暂存在的中间状态，并且先于通常观察到的总体肿胀和空泡化。
X-ray diffraction was used to probe the effects of triethyl tin (TET) on the periodicity and amount of membrane disorientation in the lamellar myelin from respiring optic and sciatic nervesin vitroas well as from nerves of rats treatedin vivothrough their drinking water. The diffraction patterns show thatin vitroTET at concentrations of 4–100 μm affects C.N.S. but not P.N.S. myelin structure. A planar, concentric membrane array with a 200 Å period is detected in the C.N.S.; this ordered, swollen myelin contrasts with the vacuolar and vesicular structure seen in thin-sections in TET-induced oedema. No effects of short-termin vivotreatment with TET are observed in either the C.N.S. or P.N.S. The finding that carbonic anhydrase (CA) inhibitors have no effect on the TET-induced structural changes indicates that the swelling we observe is not related to a CA-dependent process. In comparison, the TET effect is prevented by replacing the mobile ions with isotonic sucrose. We conclude that TET-induced swelling in C.N.S. myelin arises from an increase in ion transport followed by obligatory fluid movement. Further, the ordered, swollen structure we detect may be an intermediate state that exists transientlyin vivoin TET intoxication and that precedes the gross swelling and vacuolization usually observed.