Seasonal variations in the levels of PAH-DNA adducts in young adults living in Mexico City
Seasonal variations in the levels of PAH-DNA adducts in young adults living in Mexico City
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DOI：
10.1093/mutage/geq104
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发表时间：
2011-05-01
期刊：
影响因子：
2.7
通讯作者：
Gonsebatt, M. E.
中科院分区：
文献类型：
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作者：
Garcia-Suastegui, W. A.;Huerta-Chagoya, A.;Gonsebatt, M. E.
多环芳烃 (PAH) 是污染空气中普遍存在的成分。墨西哥城都市区（MCMA）是世界上人口最稠密的地区之一，海拔2240米。在这个海拔高度，可用氧气较少，燃烧效率较低，因此会产生更多的多环芳烃污染物。根据执行环境监测的墨西哥城自动监测网络（RAMA，西班牙语缩写；http://www.sma.df.gob.mx/simat2/informaciontecnica/index.php?opcion=5&opciondifusion_bd=90）的数据，墨西哥城的关键空气污染物是臭氧和颗粒物（PM）。 PM 排放量在旱季（冬季至春季）期间增加，在雨季（夏季至秋季）期间减少。一些 PAH 的生物活性会产生与 DNA 结合的反应性代谢物，血液淋巴细胞等组织中 PAH-DNA 加合物水平升高意味着患癌症的风险升高。我们使用一组匹配的外周血淋巴细胞，比较了 PAH-DNA 加合物的水平和染色体畸变 (CWA) 细胞的百分比，这些外周血淋巴细胞是在 2006 年旱季和随后的雨季期间两次从 MCMA 的年轻非吸烟居民 (n = 92) 中获得的。使用 r7, t8-二羟基-t-9, 10-氧基-7,8,9,10-四氢苯并[a]芘 (BPDE)-DNA 化学发光免疫分析 (CIA) 分析 PAH-DNA 加合物。 CWA 的百分比是在来自同一个体的培养淋巴细胞中测定的。测试了 DNA 加合物水平和染色体畸变与生活方式和细胞色素 P450 CYP1A1 和 CYP1B1 以及谷胱甘肽-S-转移酶 GSTM1 和 GSTT1 多态性的相关性。 PAH-DNA 加合物的水平在旱季（10.66 +/- 3.05 每 10(9) nt，n = 92）显着高于雨季（9.50 +/- 2.85 每 10(9) nt，n = 92），并且与直径为
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous components of polluted air. The Mexico City Metropolitan Area (MCMA), one of the most densely populated areas in the world, is 2240 m above sea level. At this altitude, less oxygen is available, making combustion less efficient and therefore producing more PAH pollutants. According to the Automatic Monitoring Network in Mexico City (RAMA, for its Spanish initials; http://www.sma.df.gob.mx/simat2/informaciontecnica/index.php?opcion=5&opciondifusion_bd=90), which performs environmental monitoring, the critical air pollutants in Mexico City are ozone and particulate matter (PM). PM emissions increase during the dry season (winter to spring) and decrease during the rainy season (summer to autumn). The bioactivation of some PAHs produces reactive metabolites that bind to DNA, and the presence of elevated levels of PAH-DNA adducts in tissues such as blood lymphocytes represents an elevated risk for the development of cancer. We have compared the levels of PAH-DNA adducts and the percentage of cells with chromosomal aberrations (CWAs) using a matched set of peripheral blood lymphocytes obtained on two separate occasions from young non-smoking inhabitants of the MCMA (n = 92) during the 2006 dry season and the following rainy season. PAH-DNA adducts were analysed using the r7, t8-dihydroxy-t-9, 10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (BPDE)-DNA chemiluminescence immunoassay (CIA). The percentages of CWA were determined in cultured lymphocytes from the same individuals. Both DNA adduct levels and chromosomal aberrations were tested for correlation with lifestyle and the polymorphisms of cytochromes P450 CYP1A1 and CYP1B1 as well as glutathione-S-transferases GSTM1 and GSTT1. The levels of PAH-DNA adducts were significantly higher (P < 0.001) in the dry season (10.66 +/- 3.05 per 10(9) nt, n = 92) than during the rainy season (9.50 +/- 2.85 per 10(9) nt, n = 92) and correlated with the seasonal levels of particulate matter with a diameter of