Enhancement of the LH surge by male olfactory signals is associated with AVPV Kiss1 cell activation in female rats.
Enhancement of the LH surge by male olfactory signals is associated with AVPV Kiss1 cell activation in female rats.
复制标题
雄性嗅觉信号增强 LH 激增与雌性大鼠 AVPV Kiss1 细胞激活有关。
DOI：
10.1111/jne.12505
复制
发表时间：
2017
影响因子：
3.2
通讯作者：
Tsukamura H. Inoue N.
中科院分区：
文献类型：
--
作者：
Watanabe Y;Ikegami K;Ishigaki R;Ieda N;Uenoyama Y;Maeda KI;Tsukamura H. Inoue N.
嗅觉刺激在哺乳动物的生殖功能调节中起着重要作用。本研究观察了雄性大鼠嗅觉信号对雌性大鼠Kispeptin神经元活动和黄体生成素(LH)分泌的影响。Wistar-imamichi品系雌性大鼠去卵巢后植入17β-雌二醇(E_2)。OVX+E2大鼠在暴露于清洁寝具、雌性污秽寝具或雄性污秽寝具1小时后处死。原位杂交和c-Fos免疫组织化学双重染色显示，OVX+E2组大鼠前腹侧脑室周围核(AVPV)内Kiss1表达细胞数和c-Fos免疫阳性细胞数均显著高于其他两组。弓状核c-Fos免疫阳性Kiss1阳性细胞数和c-Fos免疫阳性GnRH1阳性细胞数两组间差异无统计学意义。暴露于雄性卧铺的OVX+E2大鼠，在由终纹床核、杏仁皮质和杏仁内侧核等多个核团组成的边缘系统中，c-Fos免疫阳性细胞的数量也显著高于其他两组。染有雄性污物的OVX+E2组大鼠出现明显的黄体生成素峰值，其峰值和曲线下面积均显著高于其他两组。这些结果表明，来自雄性大鼠的嗅觉信号可能通过边缘系统激活AVPV kisspeptin神经元，导致雌性大鼠的黄体生成素峰增强。综上所述，本研究的结果表明，AVPV kispeptin神经元是调节雌性大鼠黄体生成素释放的嗅觉信号的靶标。
Olfactory stimuli play an important role in regulating reproductive functions in mammals. The present study investigated the effect of olfactory signals derived from male rats on kisspeptin neuronal activity and luteinising hormone (LH) secretion in female rats. Wistar‐Imamichi strain female rats were ovariectomised (OVX) and implanted with preovulatory levels of 17β‐oestradiol (E2). OVX+E2rats were killed 1 hour after exposure to either: clean bedding, female‐soiled bedding or male‐soiled bedding. Dual staining forKiss1mRNAin situhybridisation and c‐Fos immunohistochemistry revealed that the numbers ofKiss1‐expressing cells and c‐Fos‐immunopositiveKiss1‐expressing cells in the anteroventral periventricular nucleus (AVPV) were significantly higher in OVX+E2rats exposed to male‐soiled bedding than those of the other groups. No significant difference was found with respect to the number of c‐Fos‐immunopositiveKiss1‐expressing cells in the arcuate nucleus and c‐Fos‐immunopositiveGnrh1‐expressing cells between the groups. The number of c‐Fos‐immunopositive cells was also significantly higher in the limbic system consisting of several nuclei, such as the bed nucleus of the stria terminalis, the cortical amygdala and the medial amygdala, in OVX+E2rats exposed to male‐soiled bedding than the other groups. OVX+E2rats exposed to male‐soiled bedding showed apparent LH surges, and the peak of the LH surge and area under the curve of LH concentrations in the OVX+E2group were significantly higher than those of the other two groups. These results suggest that olfactory signals derived from male rats activate AVPV kisspeptin neurones, likely via the limbic system, resulting in enhancement of the peak of the LH surge in female rats. Taken together, the results of the present study suggests that AVPV kisspeptin neurones are a target of olfactory signals to modulate LH release in female rats.