SSU1-R, a sulfite resistance gene of wine yeast, is an allele of SSU1 with a different upstream sequence
SSU1-R, a sulfite resistance gene of wine yeast, is an allele of SSU1 with a different upstream sequence
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DOI：
10.1016/s0922-338x(98)80146-3
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Hara, S
中科院分区：
文献类型：
--
作者：
Goto-Yamamoto, N;Kitano, K;Hara, S
从从葡萄酒中分离到的高亚硫酸盐抗性酿酒酵母Y-9菌株中克隆了一个亚硫酸盐抗性基因SSU1-R。SSU1-R的编码区序列与SSU1的编码区序列几乎相同，SSU1已被证明与亚硫酸盐抗性有关。虽然SSU1位于酿酒酵母的XVI染色体上，但SSU1-R的上游区域与第VIII染色体序列同源。在我们的Northern分析中，在Y-9和K1-V1116菌株中发现了一条强烈的条带，这两株菌株都表现出很强的亚硫酸盐抗性，无论有没有亚硫酸盐。另一方面，在相同的实验条件下，对亚硫酸盐抗性较弱的菌株OC-2中没有检测到条带。对SSU1/SSU1-R的物理定位表明，该序列位于Y-9和K1-V1116、WE14两个品系的染色体上。OC-2和其他23种葡萄酒、清酒、啤酒、烧酒(日本蒸馏酒)、酒精、烘焙和实验室菌株在第16号染色体上都有这个序列，另外4个葡萄酒菌株在两条染色体上都有这个序列。因此，SSU1/SSU1-R上游序列的差异似乎导致了转录速率和亚硫酸盐抗性程度的差异。
A sulfite resistance gene, SSU1-R, was cloned from at highly sulfite-resistant strain of Saccharomyces cerevisiae, Y-9, which was isolated as a contaminant from a wine must. The coding region sequence of SSU1-R was almost identical to that of SSU1, the gene that has been shown to be responsible for sulfite resistance. Although SSU1 is located on chromosome XVI of S. cerevisiae, the upstream region of SSU1-R was homologous with a sequence of chromosome VIII. In our Northern analysis, an intense band was detected in Y-9 and K1-V1116 strains, both of which exhibit strong sulfite resistance, with and without sulfite in the medium. On the other hand, under the same experimental conditions, no band was detected in OC-2, a strain that exhibits weak sulfite resistance. Physical mapping of SSU1/SSU1-R showed that this sequence was on chromosome VIII of Y-9 and the two wine strains, K1-V1116 and WE14. OC-2 and twenty-three other wine, sake, beer, shochu (Japanese distilled liquor), alcohol, bakery, and laboratory strains, had this sequence on chromosome XVI, and four other wine strains had it on both chromosomes. Thus, the difference in the upstream sequence of SSU1/SSU1-R seems to cause differences in the transcription rates and degree of sulfite resistance.