Purification of the trehalase GMTRE1 from soybean nodules and cloning of its cDNA.: GMTRE1 is expressed at a low level in multiple tissues
Purification of the trehalase GMTRE1 from soybean nodules and cloning of its cDNA.: GMTRE1 is expressed at a low level in multiple tissues
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DOI：
10.1104/pp.119.2.489
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发表时间：
1999-02-01
期刊：
影响因子：
7.4
通讯作者：
Wiemken, A
中科院分区：
文献类型：
--
作者：
Aeschbacher, RA;Müller, J;Wiemken, A
海藻糖（α - d -glucopyranosyl-1,1- α - d -glucopyranoside）是一种在微生物和低等无脊椎动物中广泛存在的双糖，一般认为在高等植物中不存在。然而，最近发现的拟南芥基因的产物参与海藻糖的合成，重新引起了人们对海藻糖在高等植物中功能的可能性的兴趣。我们之前的研究表明，海藻糖酶（一种降解海藻糖的酶）存在于大豆根瘤中（Glycine max [L.]）。[Merr.])，我们将这种酶定性为一种外质体糖蛋白。本文描述了该海藻酶的纯化及编码该酶的全长cDNA的克隆，命名为GMTRE1 （G. max海藻酶1）。从GMTRE1的开放阅读框中获得的氨基酸序列与细菌、真菌和动物中已知的海藻酸酶具有很强的同源性。GMTRE1是一个单拷贝基因，在许多组织中以低但恒定的水平表达。
Trehalose (alpha-D-glucopyranosyl-1,1-alpha-D-glucopyranoside), a disaccharide widespread among microbes and lower invertebrates, is generally believed to be nonexistent in higher plants. However, the recent discovery of Arabidopsis genes whose products are involved in trehalose synthesis has renewed interest in the possibility of a function of trehalose in higher plants. We previously showed that trehalase, the enzyme that degrades trehalose, is present in nodules of soybean (Glycine max [L.] Merr.), and we characterized the enzyme as an apoplastic glycoprotein. Here we describe the purification of this trehalase to homogeneity and the cloning of a full-length cDNA encoding this enzyme, named GMTRE1 (G. max trehalase 1). The amino acid sequence derived from the open reading frame of GMTRE1 shows strong homology to known trehalases from bacteria, fungi, and animals. GMTRE1 is a single-copy gene and is expressed at a low but constant level in many tissues.