Affinity-based microdialysis sampling using heparin for in vitro collection of human cytokines.
Affinity-based microdialysis sampling using heparin for in vitro collection of human cytokines.
复制标题
使用肝素进行基于亲和力的微透析采样，用于体外收集人类细胞因子。
DOI：
10.1016/j.aca.2009.08.009
复制
发表时间：
2009
影响因子：
6.2
通讯作者：
Stenken,JulieA
中科院分区：
文献类型：
--
作者：
Wang,Yuexi;Stenken,JulieA
Microdialysis sampling is a widely used method to sample from complex biological matrices. Cytokines are important signaling proteins that are typically recovered with low relative recovery values during microdialysis sampling. Heparin was included in the microdialysis perfusion fluid as an affinity agent to increase in vitro recovery of different cytokines through polyethersulfone (PES) microdialysis membranes with 100kDa molecular weight cutoff. No change in fluid volumes collected from the microdialysis probes occurred when heparin was included in the perfusion fluid up to concentrations of 10μM. The loss of heparin (10μM) across the dialysis membrane was minimal (2.7±0.9%, n=3). Additionally, heparin at these concentrations did not interfere with the cytokine immunoassays. The control and heparin-enhanced relative recoveries for five human cytokines using 0.1μM heparin in the microdialysis perfusion fluid flowing at 0.5μLmin−1were (n=3): interleukin-4 (IL-4), 4.2±0.5% and 7.2±3.1%; interleukin-6 (IL-6), 1.4±0.8% and 3.6±1.3%; interleukin-7 (IL-7), 1.3±0.8% and 4.8±1.8%; monocyte chemoattractant protein-1 (MCP-1), 9.0±1.6% and 19.5±2.7%; and tumor necrosis factor-α (TNF-α), 7.4±1.3% and 16.9±1.6%, respectively. Heparin increased the microdialysis sampling relative recovery of several human cytokines in vitro.
