Hertwig's epithelial root sheath differentiation and initial cementum and bone formation during long-term organ culture of mouse mandibular first molars using serumless, chemically-defined medium.
Hertwig's epithelial root sheath differentiation and initial cementum and bone formation during long-term organ culture of mouse mandibular first molars using serumless, chemically-defined medium.
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DOI：
10.1111/j.1600-0765.1989.tb00854.x
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发表时间：
1989
影响因子：
3.5
通讯作者：
Harold C. Slavkin;P. Bringas;C. Bessem;V. Santos;Masanori Nakamura;Mingyoun Hsu;Malcolm L. Snead;M. Zeichner‐David;A. Fincham
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文献类型：
--
作者：
Harold C. Slavkin;P. Bringas;C. Bessem;V. Santos;Masanori Nakamura;Mingyoun Hsu;Malcolm L. Snead;M. Zeichner‐David;A. Fincham
研究旨在验证赫特维希上皮根鞘（HERS）合成并分泌参与无细胞牙骨质形成过程的牙釉质相关蛋白的假设。我们的实验策略是研究连续的根发育的小鼠下颌第一磨牙在体内和长期器官培养在体外使用无血清，化学成分确定的培养基。使用抗釉原蛋白，抗釉蛋白和抗肽抗体，釉质相关抗原定位在中间牙骨质在HERS分化和根形成在体内。在器官培养中保持长达31天的帽状期磨牙表达了由牙冠和初始根、牙骨质和骨形成鉴定的形态发生和细胞分化。代谢标记的HERS产品进行了分析，免疫检测使用牙釉质相关抗体和一维和二维SDS凝胶电泳。在小鼠牙骨质形成过程中发现了一种分子量为72 kDa和26 kDa的多肽。这两种根推定牙骨质蛋白质产生类似的（相同的）氨基酸组成，但是，这两种蛋白质的组合物不同的小鼠冠釉蛋白或釉原蛋白。这种方法提供了一个新的和新颖的体外模型，了解HERS分化和功能相关的根和骨形成。这些数据支持HERS细胞合成与典型冠釉蛋白相关但也不同的多肽的假设。
Studies were designed to test the hypothesis that Hertwig's epithelial root sheath (HERS) synthesizes and secretes enamel-related proteins that participate in the process of acellular cementum formation. Our experimental strategy was to examine sequential root development of the mouse mandibular first molar in vivo and in long-term organ culture in vitro using serumless, chemically-defined medium. Using anti-amelogenin, anti-enamelin and anti-peptide antibodies, enamel-related antigens were localized within intermediate cementum during HERS differentiation and root formation in vivo. Cap stage molars maintained for periods of up to 31 days in organ culture expressed morphogenesis and cytodifferentiation as identified by tooth crown and initial root, cementum and bone formation. Metabolically-labeled HERS products were analyzed by immunodetection using enamel-related antibodies and one- and two-dimensional SDS gel electrophoresis. A 72 kDa and 26 kDa polypeptide were identified in forming mouse cementum. Both of these root putative cementum proteins yield similar (identical) amino acid compositions; however, both proteins differed from the compositions of either mouse crown enamelin or amelogenin proteins. This approach provides a new and novel in vitro model towards understanding HERS differentiation and functions related to root and bone formation. The data support the hypothesis that HERS cells synthesize polypeptides related to but also different from canonical crown enamel proteins.