Serological cross-reactivity between spotted fever and typhus groups of rickettsia infection in Japan
Serological cross-reactivity between spotted fever and typhus groups of rickettsia infection in Japan
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DOI：
10.1016/j.ijid.2023.03.012
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发表时间：
2023-03-29
影响因子：
8.4
通讯作者：
Kurita, Noriaki
中科院分区：
文献类型：
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作者：
Aita, Tetsuro;Sando, Eiichiro;Kurita, Noriaki
目的：我们研究了日本斑点热（JSF）患者对伤寒立克次体交叉反应的频率，并使用抗体终点滴度评价了两种立克次体之间的差异。研究方法：在日本两个立克次体病参考中心，采用间接免疫过氧化物酶法测定了两个阶段患者抗日本立克次体和伤寒立克次体的免疫球蛋白（IG）M和IgG滴度。交叉反应定义为对R.在符合JSF诊断标准的患者中，恢复期血清中的伤寒比急性血清中的伤寒更明显。同时检测IgM和IgG的频率。结果：约20%的病例出现阳性交叉反应。抗体滴度的比较显示，难以确定一些阳性病例。结论：血清学诊断中20%的交叉反应可能导致立克次体病的分型错误。然而，除了某些情况下，我们能够成功区分JSF从鼠斑疹伤寒使用每个终点滴度。(c)2023作者（S）由爱思唯尔有限公司代表国际传染病学会出版。这是CC BY许可下的开放获取文章（http://creativecommons.org/licenses/by/4.0/）
Objectives: We examined the frequency of cross-reactions to Rickettsia typhi in patients with Japanese spotted fever (JSF) and evaluated the differences between two rickettsiae using antibody endpoint titers. Methods: Patients' immunoglobulin (Ig)M and IgG titers against Rickettsia japonica and Rickettsia typhi in two phases were measured using an indirect immunoperoxidase assay at two reference centers for rick-ettsiosis in Japan. Cross-reaction was defined as a higher titer against R. typhi in convalescent sera than in acute sera among patients fulfilling the criteria for JSF diagnosis. The frequencies of IgM and IgG were also evaluated. Results: Approximately 20% of cases showed positive cross-reactions. A comparison of antibody titers revealed the difficulty in identifying some positive cases. Conclusion: Cross-reactions of 20% in serodiagnosis may lead to the misclassification of rickettsial dis-eases. However, with the exception of some cases, we were able to successfully differentiate JSF from murine typhus using each endpoint titer. (c) 2023 The Author(s). Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/ )