Cell-surface molecules that characterize different stages in the development of cerebellar interneurons.
Cell-surface molecules that characterize different stages in the development of cerebellar interneurons.
复制标题
表征小脑中间神经元发育不同阶段的细胞表面分子。
DOI：
10.1101/sqb.1983.048.01.078
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Levine,J
中科院分区：
文献类型：
--
作者：
Stallcup,WB;Beasley,L;Levine,J
方法细胞培养和原代培养。这些研究中使用的所有克隆细胞系均来源于亚硝基乙基脲诱导的大鼠中枢神经系统肿瘤（Schubert et al. 1974）。将这些细胞系维持在补充有10%胎牛血清的Duibecco改良Eagle培养基（DME）中，如前所述制备胚胎大鼠脑和出生后大鼠小脑的原代培养物（Stallcup 1981）。将培养物维持在补充有10%胎牛血清和25 mM KCl的DME中，并在接种后5 - 21天用于免疫组织化学实验。
METHODSCell Hnes and primary cultures. All clonal cell lines used in these studies were derived from nitrosoethylurea-induced tumors of the rat central nervous system (Schubert et al. 1974). These cell lines were maintained in Duibecco's modified Eagle's medium (DME) supplemented with 10% fetal bovine serum.Primary cultures of embryonic rat brain and postnatal rat cerebellum were prepared as described previously (Stallcup 1981). Cultures were maintained in DME supplemented with 10% fetal bovine serum and 25 mM KCI and were used for immunohistochemical experiments between 5 and 21 days after plating.