Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma
Isolation and characterization of tumorigenic, stem-like neural precursors from human glioblastoma
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DOI：
10.1158/0008-5472.can-04-1364
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发表时间：
2004-10-01
期刊：
影响因子：
11.2
通讯作者：
Vescovi, A
中科院分区：
文献类型：
--
作者：
Galli, R;Binda, E;Vescovi, A
已从一些人类癌症中分离出转化的干细胞。我们报道，与其他脑癌不同，致命的多形性胶质母细胞瘤含有具备神经干细胞所预期的所有关键特征的神经前体细胞。与它们对应的正常神经干细胞相似但不完全相同，这些前体细胞在体内表现为单能（星形胶质细胞），在培养中表现为多能（神经元 - 星形胶质细胞 - 少突胶质细胞）。更重要的是，这些细胞可作为肿瘤起始细胞，直至克隆水平，并且能够建立与人类疾病的主要组织学、细胞学和结构特征非常相似的肿瘤，即使经过连续移植也是如此。因此，具有肿瘤神经干细胞所预期的所有特征的细胞似乎参与了成人多形性胶质母细胞瘤的生长和复发。
Transformed stem cells have been isolated from some human cancers. We report that, unlike other brain cancers, the lethal glioblastoma multiforme contains neural precursors endowed with all of the critical features expected from neural stem cells. Similar, yet not identical, to their normal neural stem cell counterpart, these precursors emerge as unipotent (astroglial) in vivo and multipotent (neuronal-astroglial-oligodendroglial) in culture. More importantly, these cells can act as tumor-founding cells down to the clonal level and can establish tumors that closely resemble the main histologic, cytologic, and architectural features of the human disease, even when challenged through serial transplantation. Thus, cells possessing all of the characteristics expected from tumor neural stem cells seem to be involved in the growth and recurrence of adult human glioblastomas multiforme.