Analysis of hen egg white lysozyme adsorption on Si(Ti)O2 | aqueous solution interfaces at low ionic strength:: a biphasic reaction related to solution self-association
Analysis of hen egg white lysozyme adsorption on Si(Ti)O2 | aqueous solution interfaces at low ionic strength:: a biphasic reaction related to solution self-association
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DOI：
10.1016/s0927-7765(99)00085-5
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发表时间：
2000-02-15
影响因子：
5.8
通讯作者：
Ramsden, JJ
中科院分区：
文献类型：
--
作者：
Ball, V;Ramsden, JJ
用光波导光模光谱法测量了浓度为10（-7）、10（-6）、10（-4）M和pH为8.0的鸡蛋白色溶菌酶在Si_（0.8）Ti_（0.2）O_2表面上的吸附动力学，发现在两种不同的动力学状态下进行：一个快速和准线性增加的表面浓度，其次是较慢的动力学，可以定性地描述随机顺序吸附的理论。这两个动力学制度之间的过渡与突然变化的平均折射率和吸附层的厚度的值，以及在解吸动力学之前或之后进行的过渡从第一制度到第二。在相同的离子强度和不同的pH值下进行的实验表明，溶菌酶在高浓度溶液中的吸附行为与其自缔合性质有关。(C)2000 Elsevier Science B. V.保留所有权利。
Adsorption kinetics of hen-egg white lysozyme at concentrations of 10(-7), 10(-6), 10(-4) M and at pH 8.0 have been measured on Si0.8Ti0.2O2 surfaces by means of optical waveguide lightmode spectroscopy and were found to proceed in two distinct kinetic regimes: a fast and quasi linear increase of the surface concentration followed by slower kinetics that could be described qualitatively by the theory of random sequential adsorption. The transition between these two kinetic regimes was correlated with abrupt changes in the values of the mean refractive index and of the thickness of the adsorbed layer as well as in the desorption kinetics performed either before or after the transition from the first regime to the second. Experiments carried out at the same ionic strength but at different pH values showed that the adsorption behaviour of lysozyme is related to its self-association properties in highly concentrated solutions. (C) 2000 Elsevier Science B.V. All rights reserved.