Lipid analysis of flat-mounted eye tissue by imaging mass spectrometry with identification of contaminants in preservation
Lipid analysis of flat-mounted eye tissue by imaging mass spectrometry with identification of contaminants in preservation
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DOI：
10.1007/s00216-011-5044-x
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发表时间：
2011-07-01
影响因子：
4.3
通讯作者：
Yost, Richard A.
中科院分区：
文献类型：
--
作者：
Garrett, Timothy J.;Menger, Robert F.;Yost, Richard A.
基质辅助激光解吸/电离成像质谱法用于分析供体眼组织标本的磷脂含量，以评估脂质分布。使用2，5-二羟基苯甲酸作为基质，在正离子模式下检测磷脂酰胆碱和鞘磷脂。在本研究期间，检测到较低m/z区域（小于m/z 400）中的未知离子信号，主要在人平板组织的远周，但在以类似方式制备的年龄匹配的恒河猴组织中未检测到。未知离子信号出现在m/z 304、332、360和388处。直接从组织样本中对这些离子进行串联质谱分析，并制备浸提液的精确质量测量值，用于进一步鉴定。这些离子被鉴定为烷基二甲基苄基铵表面活性剂（苯扎氯铵（BAC））。通过将眼组织提取物与含有BAC的非处方眼部护理产品进行比较，验证了这些菌种的分类。
Matrix-assisted laser desorption/ionization imaging mass spectrometry was used to analyze donor eye tissue specimens for phospholipid content to evaluate lipid distribution. Phosphatidylcholines and sphingomyelins were detected in the positive ion mode using 2,5-dihydroxybenzoic acid as the matrix. During this study, unknown ion signals in the lower m/z region (less than m/z 400) were detected, mainly in the far periphery of human flat-mounted tissue but not in age-matched rhesus monkey tissue prepared in a similar manner. The unknown ion signals occurred at m/z 304, 332, 360, and 388. These ions were subjected to tandem mass spectrometry directly from the tissue sample, and exact mass measurements of extracts were prepared for further identification. These ions were identified as alkyl dimethylbenzylammonium surfactants (benzalkonium chlorides (BACs)). The classification of these species was verified by comparing an eye tissue extract to an over-the-counter eye-care product containing BACs.