A series of Cre-ER(T2) drivers for manipulation of the skeletal muscle lineage.
A series of Cre-ER(T2) drivers for manipulation of the skeletal muscle lineage.
复制标题
一系列用于操纵骨骼肌谱系的 Cre-ER(T2) 驱动程序。
DOI：
10.1002/dvg.22792
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Lepper,Christoph
中科院分区：
文献类型：
--
作者：
Southard,Sheryl;Low,SiewHui;Li,Lydia;Rozo,Michelle;Harvey,Tyler;Fan,Chen-Ming;Lepper,Christoph
我们报道了用他莫昔芬诱导的Cre(Cre-ERT2；CE)基因盒敲入Pax3、Myod1、Myog、Myf6和Myl1的5个小鼠品系，作为骨骼肌研究社区的资源。我们使用Cre报告小鼠R26RLacZ在胚胎发育期间对这些eCEs菌株进行了表征，结果表明，它们在每个生肌基因确定的预期细胞谱系内引导严格控制的他莫昔芬诱导的报告表达。我们还检查了几个选定的成人骨骼肌群中他莫昔芬诱导的报告表达。这些新的CE等位基因都没有直接在心肌中表达报告基因。所有这些等位基因都遵循相同的敲入策略，用CEcassette替换每个基因的第一个外显子，使它们成为内源基因的空等位基因。讨论了这种设计的优点和缺点。虽然我们描述了这些菌株的潜在立即使用，但它们的用途可能超出了骨骼肌生物学中可预见的问题。创世纪52：759-770,2014。©2014 Wiley期刊，Inc.
We report the generation of five mouse strains with the tamoxifen‐inducible Cre (Cre‐ERT2; CE) gene cassette knocked into the endogenous loci ofPax3,Myod1,Myog,Myf6, andMyl1, collectively as a resource for the skeletal muscle research community. We characterized theseCEstrains using the Cre reporter mice,R26RLacZ, during embryogenesis and show that they direct tightly controlled tamoxifen‐inducible reporter expression within the expected cell lineage determined by each myogenic gene. We also examined a few selected adult skeletal muscle groups for tamoxifen‐inducible reporter expression. None of these newCEalleles direct reporter expression in the cardiac muscle. All these alleles follow the same knock‐in strategy by replacing the first exon of each gene with theCEcassette, rendering them null alleles of the endogenous gene. Advantages and disadvantages of this design are discussed. Although we describe potential immediate use of these strains, their utility likely extends beyond foreseeable questions in skeletal muscle biology. genesis 52:759–770, 2014. © 2014 Wiley Periodicals, Inc.