ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE
ELECTROPHORETIC ANALYSIS OF MAJOR POLYPEPTIDES OF HUMAN ERYTHROCYTE MEMBRANE
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DOI：
10.1021/bi00789a030
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发表时间：
1971-01-01
期刊：
影响因子：
2.9
通讯作者：
WALLACH, DFH
中科院分区：
文献类型：
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作者：
FAIRBANKS, G;STECK, TL;WALLACH, DFH
G.费尔班克斯，f.西奥多L. Steck，§ and D. F. H. Wallach/：摘要：用聚丙烯酰胺凝胶电泳法分析了人红细胞膜的多肽。六条主要条带（I-VI）共同构成了蛋白质染色图谱的三分之二以上。组分III（mol wt 89，000）在鬼膜中占主导地位;它占蛋白质的30%，数量超过106条链/鬼膜。组分I和II形成含有25%蛋白质的缓慢移动的双峰（约mol wt 250，000）。I+ II、IV（mol wt 77，500）、V（mol wt 41，300）和VI（mol wt 36，200）的摩尔量相似，落入3.4-4.6 X IO 5个链/幽灵的范围内。在用过硫酸-希夫法染色的凝胶中识别出四条带。一个广泛的希夫阳性区后面的跟踪染料对应于膜脂质。三条迁移率较低的带是唾液酸糖蛋白。其中最突出的一种具有83，500的表观分子量，并含有至少57%的鬼的唾液酸。Schiff阳性条带不被蛋白染色着色。唾液酸酶治疗鬼选择性地增加了唾液酸糖蛋白的流动性，而不影响蛋白质染色的配置文件。试图通过用各种变性剂处理从大多肽产生亚基是不成功的。正常情况下，没有看到小于15，000的多肽
G. Fairbanks, f Theodore L. Steck, § and D. F. H. Wallach/: abstract: The polypeptides of the human erythrocyte mem-brane were analyzed by polyacrylamide gel electrophoresis in1% sodium dodecyl sulfate. Six major bands (I-VI) together make up over two-thirds of the protein staining profile. Component III (mol wt89, 000) predominates in the ghost mem-brane; it constitutes 30% of the protein and numbers over 106 chains/ghost. Components I and II form a slow-moving doublet (approximate mol wt250, 000) containing 25% of the protein. The molar amounts of I+ II, IV (mol wt 77,500), V (mol wt 41,300), and VI (mol wt 36,200) are similar, falling in the range 3.4-4.6 X 105 chains/ghost. Four bands were recognized in gels stained by the periodicacid-Schiff procedure. A broad Schiff-positive zone just behind the tracking dye corresponds to membrane lipids. Three bands of lower mobil-ity are sialoglycoproteins. The most prominent of these has an apparent molecular weight of 83,500 andcontains at least 57% of the sialic acid of ghosts. The Schiff-positive bands were not colored by protein stains. Sialidase treatment of ghosts selectively increased the mobilities of the sialoglycoproteins without affecting the protein-staining profile. At-tempts to produce subunits from the large polypeptides by treatment with various denaturing agents were unsuccessful. Normally, no polypeptides of size less than 15,000 were seen