INHIBITORY EFFECTS OF TUMOR NECROSIS FACTOR-ALPHA ON FRACTURE-HEALING IN RATS
INHIBITORY EFFECTS OF TUMOR NECROSIS FACTOR-ALPHA ON FRACTURE-HEALING IN RATS
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DOI：
10.1016/8756-3282(89)90078-1
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发表时间：
1989-01-01
期刊：
影响因子：
4.1
通讯作者：
ONO, K
中科院分区：
文献类型：
--
作者：
HASHIMOTO, J;YOSHIKAWA, H;ONO, K
骨折愈合涉及一系列生物组织反应，可能受到各种生化物质的影响。这些物质之一是肿瘤坏死因子α（TNF）。本文研究了肿瘤坏死因子（TNF）对大鼠肋骨骨折愈合的影响。骨折后每天给予重组人TNF（每天400 μ g/kg体重，腹膜内）抑制骨折愈合。第20天的愈合率在TNF治疗组（4/18，22.2%）中显著低于对照组（14/18，77.8%）（通过卡方检验，p < 0.001）。组织学检查显示TNF抑制软骨愈伤组织的形成。第10天，对照组的差距区和骨膜下可见软骨形成，但TNF治疗组的12个标本中有9个在差距区未观察到软骨形成。第20天，对照组骨折端新生骨愈合，但在差距区可见成熟的纤维组织，TNF治疗组未实现骨或软骨愈合。这些结果表明，TNF抑制软骨形成在骨折愈合的骨诱导的早期阶段，并建议TNF的这种效果是由于其抑制间充质细胞分化成软骨母细胞。
Fracture healing, which involves a cascade of biological tissue responses, may be affected by various biochemical substances. One of these substances is tumor necrosis factor alpha (TNF). Studies were made on the effects of TNF on healing of fractured ribs of rats. Fracture healing was inhibited by daily administration of recombination human TNF (400 .mu.g/kg body weight per day, intraperitoneally) after fracture. The rate of union on day 20 was significantly lower in the TNF-treated group (4/18, 22.2%) than in the control group (14/18, 77.8%) (p < 0.001 by Chi-square test). Histological examination showed that TNF inhibited cartilagenous callus formation. On day 10, cartilage was seen in the gap zone and under the periosteum in the control group, but no cartilage formation was observed in the gap zone in 9 of 12 specimens from the TNF-treated group. On day 20, the fracture ends were united by newly formed bone in the control group, but mature fibrous tissue was seen in the gap zone, and bony or cartilagenous union was not achieved in the TNF-treated group. These results show that TNF inhibits cartilage formation in the early phase of bone induction in fracture healing and suggest that this effect of TNF is due to its inhibition of differentiation of mesenchymal cells into chondroblasts.